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Cultivation of recalcitrant microbes: cells are alive, well and revealing their secrets in the 21st century laboratory

Leadbetter, Jared R. (2003) Cultivation of recalcitrant microbes: cells are alive, well and revealing their secrets in the 21st century laboratory. Current Opinion in Microbiology, 6 (3). pp. 274-281. ISSN 1369-5274. doi:10.1016/S1369-5274(03)00041-9.

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Any talk of the demise of in vitro cultivation as a useful mechanism for revealing many of nature’s past and present secrets appears to be unfounded and premature. The first years of this century have been as productive in the cultivation of physiologically novel, environmentally abundant and phylogenetically distinct microbes as were the first years of the 20th century. The diversity of organic and inorganic electron donors and acceptors known to be used during microbial energy metabolisms continues to grow, expanding our appreciation for the niches that may be, or historically may have been, filled by microbes in the biosphere. Either guided and instigated by, or independent of, the results of gene inventories representing diverse environmental settings, significant advances are constantly being made in the isolation of bacteria and archaea, demonstrating either strikingly rich phylogenetic diversity or significant activity and abundance in their respective environments. The potential synergisms between molecular ecological analyses and innovative in vitro growth studies are real and should be embraced, rather than treated as dueling agents in some zero-sum game.

Item Type:Article
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Leadbetter, Jared R.0000-0002-7033-0844
Additional Information:© 2003 Elsevier Science Ltd. This research was stimulated by support from the National Science Foundation (DBI-0107908) and the US Department of Agriculture (CSREES 2001-01242). I thank the greater community of Delft School, Rutgers School and all other microbiologists who continue their efforts, in earnest, to reveal the faces and environmental functions of diverse and distinctive microbes. This review is dedicated to Professor Edward R. Leadbetter on the occasion of his 45th year of contributions to the microbial cultivation literature.
Funding AgencyGrant Number
Department of AgricultureCSREES 2001-01242
Subject Keywords:AQDS, anthraquinone disulfonate; AHDS, anthrahydroquinone disulfonate; SSU, small subunit
Issue or Number:3
Record Number:CaltechAUTHORS:20130417-160243653
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Official Citation:Jared R Leadbetter, Cultivation of recalcitrant microbes: cells are alive, well and revealing their secrets in the 21st century laboratory, Current Opinion in Microbiology, Volume 6, Issue 3, June 2003, Pages 274-281, ISSN 1369-5274, 10.1016/S1369-5274(03)00041-9.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38002
Deposited On:19 Apr 2013 19:51
Last Modified:09 Nov 2021 23:32

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