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The wide-field optical sectioning of microlens array and structured illumination-based plane-projection multiphoton microscopy

Yu, Jiun-Yann and Holland, Daniel B. and Blake, Geoffrey A. and Guo, Chin-Lin (2013) The wide-field optical sectioning of microlens array and structured illumination-based plane-projection multiphoton microscopy. Optics Express, 21 (2). pp. 2097-2109. ISSN 1094-4087.

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We present a theoretical investigation of an optical microscope design that achieves wide-field, multiphoton fluorescence microscopy with finer axial resolution than confocal microscopy. Our technique creates a thin plane of excitation light at the sample using height-staggered microlens arrays (HSMAs), wherein the height staggering of microlenses generate temporal focusing to suppress out-of-focus excitation, and the dense spacing of microlenses enables the implementation of structured illumination technique to eliminate residual out-of-focus signal. We use physical optics-based numerical simulations to demonstrate that our proposed technique can achieve diffraction-limited three-dimensional imaging through a simple optical design.

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Blake, Geoffrey A.0000-0003-0787-1610
Additional Information:© 2013 Optical Society of America. Received 31 Oct 2012; revised 23 Dec 2012; accepted 26 Dec 2012; published 18 Jan 2013. We gratefully acknowledge Dr. Alexander Egner of Laser-Laboratorium Gttingen e.V. for his kind advices on algorithm development. We are indebted to Mr. Yu-Hang Chen of National Taiwan University (NTU) for his assistance with the simulations, and we thank Dr. Chien- Cheng Chen of NTU for helpful comments. The support from Ellison Medical Foundation is acknowledged.
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Ellison Medical FoundationUNSPECIFIED
Issue or Number:2
Classification Code:OCIS codes: (180.6900) Three-dimensional microscopy; (170.0110) Imaging systems; (180.4315) Nonlinear microscopy; (260.1960) Diffraction theory
Record Number:CaltechAUTHORS:20130424-100055385
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38089
Deposited By: Tony Diaz
Deposited On:03 May 2013 22:08
Last Modified:03 Oct 2019 04:54

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