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Homogenization of Pseudomonas aeruginosa PAO1 biofilms visualized by freeze-substitution electron microscopy

Guélon, T. and Hunter, R. C. and Mathias, J. D. and Deffuant, G. (2013) Homogenization of Pseudomonas aeruginosa PAO1 biofilms visualized by freeze-substitution electron microscopy. Biotechnology and Bioengineering, 110 (5). pp. 1405-1418. ISSN 0006-3592. http://resolver.caltech.edu/CaltechAUTHORS:20130425-101939882

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Abstract

A knowledge of the mechanical properties of bacterial biofilms is required to more fully understand the processes of biofilm formation such as initial adhesion or detachment. The main contribution of this article is to demonstrate the use of homogenization techniques to compute mechanical parameters of Pseudomonas aeruginosa PAO1 biofilms. For this purpose, homogenization techniques are used to analyze freeze substitution electron micrographs of the biofilm cross-sections. The concept of a representative volume element and the study about his representativeness allows us to determine the optimal size in order to analyze these biofilm images. Results demonstrate significant heterogeneities with respect to stiffness and these can be explained by varying cell density distribution throughout the bacterial biofilms. These stiffness variations lead to different mechanical properties along the height of the biofilm. Moreover, a numerical shear stress test shows the impact of these heterogeneities on the detachment process. Several modes of detachment are highlighted according to the local strain energy in the different parts of the biofilm. Knowing where, and how, a biofilm may detach will allow better prediction of accumulation and biomass detachment.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/bit.24805DOIUNSPECIFIED
http://onlinelibrary.wiley.com/doi/10.1002/bit.24805/abstractPublisherUNSPECIFIED
Additional Information:© 2012 Wiley Periodicals, Inc. Received 16 May 2012; Revision received 8 November 2012; Accepted 28 November 2012; Accepted manuscript online 13 December 2012; Article first published online: 17 Jan. 2013. This work was supported in part by the project ANR DISCO (ANR DISCO 09-SYSC-003, SYSCOMM call). The first author’s work is carried out at the French Regional Council of Auvergne. This publication only reflects the authors’ view.
Funders:
Funding AgencyGrant Number
ANR DISCO 09-SYSC-003
Subject Keywords:homogenization technique; mechanical properties; freeze-substitution electron microscopy; bacterial biofilms; detachment process
Record Number:CaltechAUTHORS:20130425-101939882
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20130425-101939882
Official Citation:Guélon, T., Hunter, R.C., Mathias, J.D. and Deffuant, G. (2013), Homogenization of Pseudomonas aeruginosa PAO1 biofilms visualized by freeze-substitution electron microscopy. Biotechnol. Bioeng., 110: 1405–1418. doi: 10.1002/bit.24805
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38114
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:29 Apr 2013 23:46
Last Modified:06 Jul 2017 15:34

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