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Imaging Proteins, Cells, and Tissues Dynamics during Embryogenesis with Two-Photon Light-Sheet Microscopy

Truong, Thai V. and Holland, Daniel B. and Trivedi, Vikas and Fraser, Scott E. (2013) Imaging Proteins, Cells, and Tissues Dynamics during Embryogenesis with Two-Photon Light-Sheet Microscopy. Biophysical Journal, 104 (2). 337A. ISSN 0006-3495. doi:10.1016/j.bpj.2012.11.1872. https://resolver.caltech.edu/CaltechAUTHORS:20130426-084318457

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Abstract

Two-photon light sheet microscopy combines nonlinear excitation with the novel sheet-illumination, orthogonal to the detection direction, to achieve high penetration depth, high acquisition speed, and low photodamage, compared with conventional imaging techniques. These advantages allow unprecedented observation of the processes that govern embryogenesis, where the ability to image fast the dynamic three dimensional structure of the developing embryo, over extended periods of time, is critical. We present a selection of applications where two-photon light sheet microscopy is utilized to observe the dynamics of proteins, cells, and tissues, toward an understanding of the construction program of the developing embryos.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.bpj.2012.11.1872DOIArticle
http://www.sciencedirect.com/science/article/pii/S0006349512031189PublisherArticle
ORCID:
AuthorORCID
Trivedi, Vikas0000-0003-0953-0553
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2013 Biophysical Society. Published by Elsevier Inc.
Issue or Number:2
DOI:10.1016/j.bpj.2012.11.1872
Record Number:CaltechAUTHORS:20130426-084318457
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130426-084318457
Official Citation:Thai V. Truong, Daniel B. Holland, Vikas Trivedi, Scott E. Fraser, Imaging Proteins, Cells, and Tissues Dynamics during Embryogenesis with Two-Photon Light-Sheet Microscopy, Biophysical Journal, Volume 104, Issue 2, Supplement 1, 29 January 2013, Page 337a, ISSN 0006-3495, 10.1016/j.bpj.2012.11.1872.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38128
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:09 May 2013 23:11
Last Modified:09 Nov 2021 23:33

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