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Activated GTPase Movement on SRP RNA Drives Cotranslational Protein Targeting

Shen, Kuang and Arslan, Sinan and Akopian, David and Ha, Taekjip and Shan, Shu-ou (2013) Activated GTPase Movement on SRP RNA Drives Cotranslational Protein Targeting. Biophysical Journal, 104 (2). 419A. ISSN 0006-3495. doi:10.1016/j.bpj.2012.11.2334.

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Signal Recognition Particle (SRP) and its receptor (SR) are co-translational protein targeting machineries responsible for delivering ribosome-nascent chain-complexes (RNCs) from cytosol to cellular membranes. Loaded with its cargo (RNC), SRP forms a complex with SR and bring the RNC to the membrane. Extensive rearrangements in this complex activate GTP hydrolysis and unload the RNC onto the translocon. During this process, the SRP RNA plays an essential role in accelerating both SRP-SR complex formation and GTP hydrolysis step. Here we show that the SRP RNA is a bi-functional molecule with its two ends, the tetraloop end and the distal end, stimulating different stages of SRP-SR interaction. using single molecule techniques to direct visualize the global relocalization along the SRP RNA, we demonstrate that the SRP-SR GTPase complex travels over 100A in the targeting reaction, from the RNA's tetraloop end during initial complex assembly to the distal end during GTPase activation. Moreover, this rearrangement is tightly regulated by the RNC and the translocon. The large-scale movement of the GTPase complex provides an attractive mechanism for coupling GTPase activation to the transfer of RNC from SRP to translocon, thereby ensuring productive protein targeting.

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Shan, Shu-ou0000-0002-6526-1733
Additional Information:© 2013 Biophysical Society. Published by Elsevier Inc. Available online 29 January 2013. 2152-Pos B171
Issue or Number:2
Record Number:CaltechAUTHORS:20130429-084737150
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Official Citation:Kuang Shen, Sinan Arslan, David Akopian, Taekjip Ha, Shu-ou Shan, Activated GTPase Movement on SRP RNA Drives Cotranslational Protein Targeting, Biophysical Journal, Volume 104, Issue 2, Supplement 1, 29 January 2013, Page 419a, ISSN 0006-3495, 10.1016/j.bpj.2012.11.2334.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38146
Deposited By: Tony Diaz
Deposited On:07 May 2013 19:33
Last Modified:09 Nov 2021 23:33

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