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Molecular and isotopic analysis of anaerobic methane-oxidizing communities in marine sediments

Hinrichs, Kai-Uwe and Summons, Roger E. and Orphan, Victoria and Sylva, Sean P. and Hayes, John M. (2000) Molecular and isotopic analysis of anaerobic methane-oxidizing communities in marine sediments. Organic Geochemistry, 31 (12). pp. 1685-1701. ISSN 0146-6380. http://resolver.caltech.edu/CaltechAUTHORS:20130429-141001775

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Abstract

Convergent lines of molecular, carbon-isotopic, and phylogenetic evidence have previously indicated (Hinrichs, K.-U., Hayes, J.M., Sylva, S.P., Brewer, P.G., DeLong, E. F., 1999. Methane-consuming archaebacteria in marine sediments. Nature 398, 802–805.) that archaea are involved in the anaerobic oxidation of methane in sediments from the Eel River Basin, offshore northern California. Now, further studies of those same sediments and of sediments from a methane seep in the Santa Barbara Basin have confirmed and extended those results. Mass spectrometric and chromatographic analyses of an authentic standard of sn-2-hydroxyarchaeol (hydroxylated at C-3 in the sn-2 phytanyl moiety) have confirmed our previous, tentative identification of this compound but shown that the previously examined product was the mono-TMS, rather than di-TMS, derivative. Further analyses of ^(13)C-depleted lipids, appreciably more abundant in samples from the Santa Barbara Basin, have shown that the archaeal lipids are accompanied by two sets of products that are only slightly less depleted in ^(13)C. These are additional glycerol ethers and fatty acids. The alkyl substituents in the ethers (mostly monoethers, with some diethers) are non-isoprenoidal. The carbon-number distributions and isotopic compositions of the alkyl substituents and of the fatty acids are similar, suggesting strongly that they are produced by the same organisms. Their structures, n-alkyl and methyl-branched n-alkyl, require a bacterial rather than archaeal source. The non-isoprenoidal glycerol ethers are novel constituents in marine sediments but have been previously reported in thermophilic, sulfate- and nitrate-reducing organisms which lie near the base of the rRNA-based phylogenetic tree. Based on previous observations that the anaerobic oxidation of methane involves a net transfer of electrons from methane to sulfate, it appears likely that the non-archaeal, ^(13)C-depleted lipids are products of one or more previously unknown sulfate-reducing bacteria which grow syntrophically with the methane-utilizing archaea. Their products account for 50% of the fatty acids in the sample from the Santa Barbara Basin. At all methane-seep sites examined, the preservation of aquatic products is apparently enhanced because the methane-oxidizing consortium utilizes much of the sulfate that would otherwise be available for remineralization of materials from the water column.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/S0146-6380(00)00106-6DOIArticle
http://www.sciencedirect.com/science/article/pii/S0146638000001066PublisherArticle
ORCID:
AuthorORCID
Orphan, Victoria0000-0002-5374-6178
Additional Information:© 2000 Elsevier Science Ltd. We are grateful to Dennis Sprott (National Research Council of Canada, Ottawa) for providing us with an authentic standard of sn-2-hydroxyarchaeol, and to Simon Brassell (Indiana University), Rich Pancost (NIOZ), and Jaap Sinninghe Damsté (NIOZ) for discussion of mass spectra. We also thank the crew of RV Point Lobos and ROV Ventana for support during sampling. This research was funded by a research fellowship to KH by the Deutsche Forschungsgemeinschaft and by support of NASA grant NAG6-6660 for laboratory expenses. Roger Summons publishes with the approval of the CEO of AGSO. This is Woods Hole Oceanographic Institution contribution 10256.
Funders:
Funding AgencyGrant Number
Deutsche Forschungsgemeinschaft (DFG)UNSPECIFIED
NASANAG6-6660
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Other Numbering System NameOther Numbering System ID
Woods Hole Oceanographic Institution Contribution10256
Record Number:CaltechAUTHORS:20130429-141001775
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20130429-141001775
Official Citation:Kai-Uwe Hinrichs, Roger E Summons, Victoria Orphan, Sean P Sylva, John M Hayes, Molecular and isotopic analysis of anaerobic methane-oxidizing communities in marine sediments, Organic Geochemistry, Volume 31, Issue 12, December 2000, Pages 1685-1701, ISSN 0146-6380, 10.1016/S0146-6380(00)00106-6.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38166
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:29 Apr 2013 22:45
Last Modified:09 Jan 2016 16:24

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