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Amber Mutants of Bacteriophage T4D: Their Isolation and Genetic Characterization

Epstein, Richard H. and Bolle, Antoinette and Steinberg, Charles M. (2012) Amber Mutants of Bacteriophage T4D: Their Isolation and Genetic Characterization. Genetics, 190 (3). pp. 833-840. ISSN 0016-6731. doi:10.1534/genetics.112.138438. https://resolver.caltech.edu/CaltechAUTHORS:20130513-153611678

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Abstract

We have isolated a large number of mutants of bacteriophage T4D that are unable to form plaques on strain B of Escherichia coli, but are able to grow (nearly) normally on some other strains of E. coli, in particular strain CR63. These mutants, designated amber (am), have been characterized by complementation tests, by genetic crosses, and by their response to chemical mutagens. It is concluded that a particular subclass of base substitution mutations may give rise to amber mutants and that such mutants occur in many genes, which are widely distributed over the T4 genome.


Item Type:Article
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URLURL TypeDescription
http://dx.doi.org/10.1534/genetics.112.138438DOIUNSPECIFIED
http://www.genetics.org/content/190/3/831PublisherUNSPECIFIED
http://resolver.caltech.edu/CaltechAUTHORS:20130513-155656517Related ItemUNSPECIFIED
Additional Information:© 2012 Genetics Society of America. This work was supported by the U.S. Public Health Service (grant E 4267) and by a U.S. Public Health Postdoctoral grant (RG-6965).
Funders:
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)E 4267
U.S. Public Health Postdoctoral GrantRG-6965
Issue or Number:3
DOI:10.1534/genetics.112.138438
Record Number:CaltechAUTHORS:20130513-153611678
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130513-153611678
Official Citation:Amber Mutants of Bacteriophage T4D: Their Isolation and Genetic Characterization Richard H. Epstein, Antoinette Bolle, and Charles M. Steinberg Genetics March 2012 190:831-840
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38473
Collection:CaltechAUTHORS
Deposited By: Jason Perez
Deposited On:13 May 2013 22:56
Last Modified:09 Nov 2021 23:37

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