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An inositolless mutant strain of neurospora and its use in bioassays

Beadle, G. W. (1944) An inositolless mutant strain of neurospora and its use in bioassays. Journal of Biological Chemistry, 156 (2). pp. 683-689. ISSN 0021-9258. https://resolver.caltech.edu/CaltechAUTHORS:20130515-104631831

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Abstract

A mutant of Neurospora crassa requiring inositol for growth is described. Its use in a bioassay method for inositol has been investigated. The suggested method appears to be satisfactory in reproducibility and precision. It is useful for concentrations between 5 and 30 γ of inositol per 20 ml. of medium. Inositol contents of a number of materials are reported, but it has not been established that these values represent both free and combined forms of inositol.


Item Type:Article
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http://www.jbc.org/content/156/2PublisherUNSPECIFIED
Additional Information:© 1944 American Society for Biochemistry and Molecular Biology. Received for publication, August 29, 1944. The work reported here was supported by grants from the Rockefeller Foundation, the Research Corporation, and the Nutrition Foundation, Inc. Mutant Strain 37401 was detected and determined as in.ositolless by Miss Janet M. Wallace. The cross between Strains 37102 and 37401 was made and analyzed by Miss Agnes J. Vincenti. The author is indebted to Dr. D. W. Woolley for a sample of lipositol and to members of the Genetics Laboratories at St.anford University for helpful suggestions.
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Funding AgencyGrant Number
Rockefeller FoundationUNSPECIFIED
Research CorporationUNSPECIFIED
Nutrition Foundation, Inc.UNSPECIFIED
Issue or Number:2
Record Number:CaltechAUTHORS:20130515-104631831
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130515-104631831
Official Citation:An Inositolless Mutant Strain of Neurospora and Its Use In Bioassays. G. W. Beadle. J. Biol. Chem. 1944 156: 683-689.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38519
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:15 May 2013 21:38
Last Modified:03 Oct 2019 04:58

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