CaltechAUTHORS
  A Caltech Library Service

A Synthetic Gene Drive System for Local, Reversible Modification and Suppression of Insect Populations

Akbari, Omar S. and Matzen, Kelly D. and Marshall, John M. and Huang, Haixa and Ward, Catherine M. and Hay, Bruce A. (2013) A Synthetic Gene Drive System for Local, Reversible Modification and Suppression of Insect Populations. Current Biology, 23 (8). pp. 671-677. ISSN 0960-9822. PMCID PMC8459379. doi:10.1016/j.cub.2013.02.059. https://resolver.caltech.edu/CaltechAUTHORS:20130531-102648320

[img] PDF - Accepted Version
See Usage Policy.

1MB
[img]
Preview
PDF - Supplemental Material
See Usage Policy.

1MB

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20130531-102648320

Abstract

Replacement of wild insect populations with genetically modified individuals unable to transmit disease provides a self-perpetuating method of disease prevention but requires a gene drive mechanism to spread these traits to high frequency. Drive mechanisms requiring that transgenes exceed a threshold frequency in order to spread are attractive because they bring about local but not global replacement, and transgenes can be eliminated through dilution of the population with wild-type individuals and 6]. These features are likely to be important in many social and regulatory contexts. Here we describe the first creation of a synthetic threshold-dependent gene drive system, designated maternal-effect lethal underdominance (UD^(MEL)), in which two maternally expressed toxins, located on separate chromosomes, are each linked with a zygotic antidote able to rescue maternal-effect lethality of the other toxin. We demonstrate threshold-dependent replacement in single- and two-locus configurations in Drosophila. Models suggest that transgene spread can often be limited to local environments. They also show that in a population in which single-locus UDMEL has been carried out, repeated release of wild-type males can result in population suppression, a novel method of genetic population manipulation.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1016/j.cub.2013.02.059DOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8459379PubMed CentralArticle
ORCID:
AuthorORCID
Akbari, Omar S.0000-0002-6853-9884
Marshall, John M.0000-0003-0603-7341
Hay, Bruce A.0000-0002-5486-0482
Additional Information:© 2013 Elsevier Ltd. Received: October 10, 2012; Revised: December 18, 2012; Accepted: February 27, 2013; Published: March 28, 2013. This work was supported by grants to B.A.H. from the National Institutes of Health (DP10D003878) and the Weston Havens Foundation and to J.M.M. from the Medical Research Council, UK.
Funders:
Funding AgencyGrant Number
NIHDP10D003878
Weston Havens FoundationUNSPECIFIED
Medical Research Council (UK)UNSPECIFIED
Issue or Number:8
PubMed Central ID:PMC8459379
DOI:10.1016/j.cub.2013.02.059
Record Number:CaltechAUTHORS:20130531-102648320
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130531-102648320
Official Citation:Omar S. Akbari, Kelly D. Matzen, John M. Marshall, Haixia Huang, Catherine M. Ward, Bruce A. Hay, A Synthetic Gene Drive System for Local, Reversible Modification and Suppression of Insect Populations, Current Biology, Volume 23, Issue 8, 22 April 2013, Pages 671-677, ISSN 0960-9822, 10.1016/j.cub.2013.02.059.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38725
Collection:CaltechAUTHORS
Deposited By:INVALID USER
Deposited On:03 Jun 2013 19:14
Last Modified:27 Sep 2021 20:38

Repository Staff Only: item control page