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The Glove-like Structure of the Conserved Membrane Protein TatC Provides Insight into Signal Sequence Recognition in Twin-Arginine Translocation

Ramasamy, Sureshkumar and Abrol, Ravinder and Suloway, Christian J. M. and Clemons, William M., Jr. (2013) The Glove-like Structure of the Conserved Membrane Protein TatC Provides Insight into Signal Sequence Recognition in Twin-Arginine Translocation. Structure, 21 (5). pp. 777-788. ISSN 0969-2126. PMCID PMC3653977. https://resolver.caltech.edu/CaltechAUTHORS:20130614-150529575

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Abstract

In bacteria, two signal-sequence-dependent secretion pathways translocate proteins across the cytoplasmic membrane. Although the mechanism of the ubiquitous general secretory pathway is becoming well understood, that of the twin-arginine translocation pathway, responsible for translocation of folded proteins across the bilayer, is more mysterious. TatC, the largest and most conserved of three integral membrane components, provides the initial binding site of the signal sequence prior to pore assembly. Here, we present two crystal structures of TatC from the thermophilic bacteria Aquifex aeolicus at 4.0 Å and 6.8 Å resolution. The membrane architecture of TatC includes a glove-shaped structure with a lipid-exposed pocket predicted by molecular dynamics to distort the membrane. Correlating the biochemical literature to these results suggests that the signal sequence binds in this pocket, leading to structural changes that facilitate higher order assemblies.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.str.2013.03.004DOIArticle
http://www.sciencedirect.com/science/article/pii/S0969212613000853PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3653977PubMed CentralArticle
ORCID:
AuthorORCID
Abrol, Ravinder0000-0001-7333-6793
Clemons, William M., Jr.0000-0002-0021-889X
Additional Information:© 2013 Elsevier Ltd. Received: November 28, 2012. Revised: February 14, 2013. Accepted: March 7, 2013. Published: April 11, 2013. We are primarily grateful to Susan Lea and Ben Berks, both at the University of Oxford, for collegiality and for sharing their unpublished structure coordinates. We thank Justin W. Chartron for help with data processing and refinement. We thank William A. Goddard III for the use of the Materials and Process Simulation Center computing clusters for MD simulations. We are grateful to the staff at SSRL beamline 12-2 and J. Kaiser for help in data collection. This long-term project has received assistance from too many people to name specifically; therefore, we are grateful to all who have contributed to this project over the years. We thank D. Rees, S. Shan, S. Tanaka, J. Chartron, G. Lin, A. Müller, V. Somalinga, E. Chun, and H. Gristick for comments on the manuscript. We thank J.T. Clemons, O.W. Clemons, and A. Sureshkumar for support. We are grateful to Gordon and Betty Moore for support of the Molecular Observatory at the California Institute of Technology. Operations at SSRL are supported by the U.S. Department of Energy and the National Institutes of Health (NIH). This work was supported by a Searle Scholar fellowship, a Burroughs-Wellcome Fund Career Award, and an NIH Pioneer Award (Grant 1DP1OD008304-01) to W.M.C.
Funders:
Funding AgencyGrant Number
Gordon and Betty Moore FoundationUNSPECIFIED
Department of Energy (DOE)UNSPECIFIED
NIHUNSPECIFIED
Searle Scholars ProgramUNSPECIFIED
Burroughs-Wellcome FundUNSPECIFIED
NIH1DP1OD008304-01
Issue or Number:5
PubMed Central ID:PMC3653977
Record Number:CaltechAUTHORS:20130614-150529575
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130614-150529575
Official Citation:Sureshkumar Ramasamy, Ravinder Abrol, Christian J.M. Suloway, William M. Clemons Jr., The Glove-like Structure of the Conserved Membrane Protein TatC Provides Insight into Signal Sequence Recognition in Twin-Arginine Translocation, Structure, Volume 21, Issue 5, 7 May 2013, Pages 777-788, ISSN 0969-2126, 10.1016/j.str.2013.03.004. (http://www.sciencedirect.com/science/article/pii/S0969212613000853)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38950
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:18 Jun 2013 16:39
Last Modified:03 Oct 2019 05:02

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