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Antibodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant Forms

Khoshnan, Ali and Ou, Susan and Ko, Jan and Patterson, Paul H. (2004) Antibodies Against Huntingtin: Production and Screening of Monoclonals and Single-Chain Recombinant Forms. In: Trinucleotide Repeat Protocols. Methods in Molecular Biology. No.277. Humana Press , Totowa, NJ, pp. 87-102. ISBN 978-1-58829-243-8. https://resolver.caltech.edu/CaltechAUTHORS:20130618-141957582

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Abstract

Antibodies can be extremely useful tools for the field of triplet repeat diseases. These reagents are important for localizing proteins in tissues, and within cells, they can be used in the isolation and characterization of the components of protein complexes, they can distinguish proteins with normal or an expanded polyglutamine repeat, they may be able to distinguish distinct conformations of a protein, and they can be used to perturb the function of proteins in living cells. Our group has produced monoclonal and recombinant single-chain antibodies that can be used for each of these purposes with huntingtin. This is the protein that, when mutated to contain an expanded polyQ motif, causes Huntington’s disease.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1385/1-59259-804-8:087DOIArticle
http://link.springer.com/protocol/10.1385%2F1-59259-804-8%3A087PublisherArticle
Alternate Title:Antibodies Against Huntingtin - Production and Screening of Monoclonals and Single-Chain Recombinant Forms
Additional Information:© 2004 Humana Press Inc. Work cited from the authors’ laboratory was supported by the Hereditary Disease Foundation.
Funders:
Funding AgencyGrant Number
Hereditary Disease FoundationUNSPECIFIED
Subject Keywords:MAb; scFv; huntingtin
Series Name:Methods in Molecular Biology
Issue or Number:277
Record Number:CaltechAUTHORS:20130618-141957582
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130618-141957582
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:38974
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Jun 2013 16:18
Last Modified:03 Oct 2019 05:02

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