Trinh, Le A. and Fraser, Scott E. (2013) Enhancer and gene traps for molecular imaging and genetic analysis in zebrafish. Development, Growth & Differentiation, 55 (4). pp. 434-445. ISSN 0012-1592. doi:10.1111/dgd.12055. https://resolver.caltech.edu/CaltechAUTHORS:20130620-152332992
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Abstract
Enhancer and gene trapping methods are highly effective means for the identification and functional analysis of transcriptionally active genes. With recent advances in fluorescent proteins and transposon based integration technologies, a growing family of trapping approaches has been developed in zebrafish, offering powerful tools to both visualize and functionally dissect gene networks during development. Coupled with the intrinsic advantages of the zebrafish model system, creative genetic engineering of trap vectors has enabled high-resolution molecular imaging and genetic manipulations. This review highlights the different enhancer and gene trap approaches that have been developed in zebrafish and offers insights into the strengths, limitations and experimental strategies for their application to enrich our knowledge of gene function and the cellular processes they control.
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| Additional Information: | © 2013 The Authors. Development, Growth & Differentiation © 2013 Japanese Society of Developmental Biologists. Received 10 January 2013; revised 4 March 2013; accepted 5 March 2013. Article first published online: 8 Apr. 2013. We thank Rusty Lansford and Masahiro Kitano for helpful comments. Work related to gene trapping in our laboratory has been supported by NHGRI Center of Excellence in Genomic Science grant P50HG004071. | |||||||||
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| Subject Keywords: | enhancer trap, gene trap, insertional mutagenesis, molecular imaging, protein trap, transposon | |||||||||
| Issue or Number: | 4 | |||||||||
| DOI: | 10.1111/dgd.12055 | |||||||||
| Record Number: | CaltechAUTHORS:20130620-152332992 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20130620-152332992 | |||||||||
| Official Citation: | Trinh, L. A. and Fraser, S. E. (2013), Enhancer and gene traps for molecular imaging and genetic analysis in zebrafish. Development, Growth & Differentiation, 55: 434–445. doi: 10.1111/dgd.12055 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 39009 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 24 Jun 2013 16:23 | |||||||||
| Last Modified: | 09 Nov 2021 23:41 |
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