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Mechanism of an ATP-independent Protein Disaggregase - I. Structure of a Membrane Protein Aggregate Reveals a Mechanism of Recognition by its Chaperone

Nguyen, Thang X. and Jaru-Ampornpan, Peera and Lam, Vinh Q. and Cao, Peigen and Piszkiewicz, Samantha and Hess, Sonja and Shan, Shu-ou (2013) Mechanism of an ATP-independent Protein Disaggregase - I. Structure of a Membrane Protein Aggregate Reveals a Mechanism of Recognition by its Chaperone. Journal of Biological Chemistry, 288 (19). pp. 13420-13430. ISSN 0021-9258. PMCID PMC3650380. https://resolver.caltech.edu/CaltechAUTHORS:20130621-085950473

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Abstract

Protein aggregation is detrimental to the maintenance of proper protein homeostasis in all cells. To overcome this problem, cells have evolved a network of molecular chaperones to prevent protein aggregation and even reverse existing protein aggregates. The most extensively studied disaggregase systems are ATP-driven macromolecular machines. Recently, we reported an alternative disaggregase system in which the 38-kDa subunit of chloroplast signal recognition particle (cpSRP43) efficiently reverses the aggregation of its substrates, the light-harvesting chlorophyll a/b-binding (LHC) proteins, in the absence of external energy input. To understand the molecular mechanism of this novel activity, here we used biophysical and biochemical methods to characterize the structure and nature of LHC protein aggregates. We show that LHC proteins form micellar, disc-shaped aggregates that are kinetically stable and detergent-resistant. Despite the nonamyloidal nature, the LHC aggregates have a defined global organization, displaying the chaperone recognition motif on its solvent-accessible surface. These findings suggest an attractive mechanism for recognition of the LHC aggregate by cpSRP43 and provide important constraints to define the capability of this chaperone.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1074/jbc.M113.462812 DOIArticle
http://www.jbc.org/content/288/19/13420PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3650380/PubMed CentralArticle
ORCID:
AuthorORCID
Hess, Sonja0000-0002-5904-9816
Shan, Shu-ou0000-0002-6526-1733
Additional Information:© 2013 by The American Society for Biochemistry and Molecular Biology, Inc. Received for publication, February 18, 2013, and in revised form, March 13, 2013 Published, JBC Papers in Press, March 22, 2013. Supported by a grant from the Betty and Gordon Moore Foundation. Supported by the David and Lucile Packard Fellowship in science and engineering, the Henry Dreyfus Teacher-Scholar Award, and the Breakthroughs in Gerontology award from the American Federation for Aging Research. We thank A. N. Murray and Dr. J. W. Kelly for recrystallized ThT and fibrilized Aβ_(1–40), A. McDowall for EM analyses, members of the Proteome Exploration Laboratory for mass spectrometry assistance, and members of the Shan group for helpful comments on the manuscript. The Proteome Exploration Laboratory is supported by the Betty and Gordon Moore Foundation and the Beckman Institute. AFM studies were supported by United States Department of Energy Grant DE-FG03-01ER46175 (to J. Heath).
Funders:
Funding AgencyGrant Number
Gordon and Betty Moore FoundationUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
Department of Energy (DOE)DE-FG03-01ER46175
David and Lucile Packard FoundationUNSPECIFIED
Camille and Henry Dreyfus FoundationUNSPECIFIED
American Federation for Aging ResearchUNSPECIFIED
Issue or Number:19
PubMed Central ID:PMC3650380
Record Number:CaltechAUTHORS:20130621-085950473
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130621-085950473
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39016
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:24 Jun 2013 16:09
Last Modified:03 Oct 2019 05:03

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