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The Analysis of Lineage-Specific Gene Activity During Sea Urchin Development

Cameron, R. Andrew and Zeller, Robert W. and Coffman, James A. and Davidson, Eric H. (1996) The Analysis of Lineage-Specific Gene Activity During Sea Urchin Development. In: Molecular Zoology: Advances, Strategies, and Protocols. Wiley-Liss , New York, pp. 221-243. ISBN 9780471144496. https://resolver.caltech.edu/CaltechAUTHORS:20130628-105154016

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Abstract

The experimental approach presented here aims to understand the mechanisms by which differential genomic expression is first established in the various spatial domains of the sea urchin embryo. In terms of regulatory organization, the sea urchin and most invertebrate taxa share a general form of embryogenesis in which cell lineage plays an important role. Regions upstream of genes whose expression patterns identify particular embryonic territories are used to construct in vitro reporter genes, which are easily injected into sea urchin embryos. Various reporter gene constructs are utilized to identify regulatory domains of the territorial marker genes. By experimentally altering the sequences in the regulatory domains, we can ascertain the functional relationship among the components. Because sea urchin embryos are easily obtained in large quantity, a straightforward strategy for the biochemical isolation of their nuclear proteins has proved successful. Techniques including affinity chromatography, gel mobility assays, and protein gel blots probed with DNA fragments permit the direct isolation and identification of proteins that specifically bind to segments of the regulatory domains. When interpreted against a background of cell lineage and fate, the mechanisms of transcriptional regulation thus revealed produce a vertically integrated explanation of blastomere specification in the early sea urchin embryo.


Item Type:Book Section
ORCID:
AuthorORCID
Cameron, R. Andrew0000-0003-3947-6041
Additional Information:© 1996 Wiley-Liss, Inc. The original research from the Davidson laboratory cited in this chapter was supported by grants from the NIH and the NSF. R.W.Z. was supported by the ONR AASERT program (N00014-93-1-1400).
Funders:
Funding AgencyGrant Number
NIHUNSPECIFIED
NSFUNSPECIFIED
Office of Naval Research (ONR)N00014-93-1-1400
Record Number:CaltechAUTHORS:20130628-105154016
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130628-105154016
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39139
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Jul 2013 15:42
Last Modified:03 Oct 2019 05:04

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