High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase

Lauchli, Ryan and Rabe, Kersten S. and Kalbarczyk, Karolina Z. and Tata, Amulya and Heel, Thomas and Kitto, Rebekah Z. and Arnold, Frances H. (2013) High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase. Angewandte Chemie International Edition, 52 (21). pp. 5571-5574. ISSN 1433-7851. PMCID PMC3828198. doi:10.1002/anie.201301362. https://resolver.caltech.edu/CaltechAUTHORS:20130701-105908055

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Abstract

The development of high-throughput assays can be extremely challenging, yet is essential for many applications in drug discovery and enzyme engineering.

Item Type:

Article

Related URLs:

URL	URL Type	Description
http://dx.doi.org/10.1002/anie.201301362	DOI	Article
http://onlinelibrary.wiley.com/doi/10.1002/anie.201301362/abstract	Publisher	Article
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828198	PubMed Central	Article

ORCID:

Author	ORCID
Arnold, Frances H.	0000-0002-4027-364X

Alternate Title:

High-throughput screening and directed evolution of terpene synthase-catalyzed cylization

Additional Information:

© 2013 Wiley-VCH Verlag GmbH & Co. Received: February 15, 2013; Published online: March 26, 2013. We are grateful to David Cane, Scott Virgil, Robert M. Coates, and David Christianson for their pioneering studies, invaluable advice, encouragement, and materials. We thank Indira Wu, Chris Farwell, and Jack Zhang for assistance. R.L. acknowledges the support of NIH fellowship F32GM095061. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. K.S.R. thanks the Deutscher Akademischer Austauschdienst (DAAD) for a postdoctoral fellowship. K.Z.K. and R.Z.K. acknowledge the support of Summer Undergraduate Research Fellowships from the California Institute of Technology. T.H. was funded by the FWF grant number: J3327-B21.

Funders:

Funding Agency	Grant Number
NIH	F32GM095061
Deutscher Akademischer Austauschdienst (DAAD)	UNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)	UNSPECIFIED
FWF Der Wissenschaftsfonds	J3327-B21

Subject Keywords:

directed evolution; high-throughput screening; protein engineering; terpenes; thermostability

Issue or Number:

PubMed Central ID:

PMC3828198

DOI:

10.1002/anie.201301362

Record Number:

CaltechAUTHORS:20130701-105908055

Persistent URL:

https://resolver.caltech.edu/CaltechAUTHORS:20130701-105908055

Official Citation:

Lauchli, R., Rabe, K. S., Kalbarczyk, K. Z., Tata, A., Heel, T., Kitto, R. Z. and Arnold, F. H. (2013), High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase . Angew. Chem. Int. Ed., 52: 5571–5574. doi: 10.1002/anie.201301362

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ID Code:

39162

Collection:

CaltechAUTHORS

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Deposited On:

01 Jul 2013 21:12

Last Modified:

09 Nov 2021 23:43

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