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High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase

Lauchli, Ryan and Rabe, Kersten S. and Kalbarczyk, Karolina Z. and Tata, Amulya and Heel, Thomas and Kitto, Rebekah Z. and Arnold, Frances H. (2013) High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase. Angewandte Chemie International Edition, 52 (21). pp. 5571-5574. ISSN 1433-7851. PMCID PMC3828198. doi:10.1002/anie.201301362. https://resolver.caltech.edu/CaltechAUTHORS:20130701-105908055

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Abstract

The development of high-throughput assays can be extremely challenging, yet is essential for many applications in drug discovery and enzyme engineering.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/anie.201301362DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/anie.201301362/abstractPublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3828198PubMed CentralArticle
ORCID:
AuthorORCID
Arnold, Frances H.0000-0002-4027-364X
Alternate Title:High-throughput screening and directed evolution of terpene synthase-catalyzed cylization
Additional Information:© 2013 Wiley-VCH Verlag GmbH & Co. Received: February 15, 2013; Published online: March 26, 2013. We are grateful to David Cane, Scott Virgil, Robert M. Coates, and David Christianson for their pioneering studies, invaluable advice, encouragement, and materials. We thank Indira Wu, Chris Farwell, and Jack Zhang for assistance. R.L. acknowledges the support of NIH fellowship F32GM095061. The content is solely the responsibility of the authors and does not necessarily represent the official views of the NIH. K.S.R. thanks the Deutscher Akademischer Austauschdienst (DAAD) for a postdoctoral fellowship. K.Z.K. and R.Z.K. acknowledge the support of Summer Undergraduate Research Fellowships from the California Institute of Technology. T.H. was funded by the FWF grant number: J3327-B21.
Funders:
Funding AgencyGrant Number
NIHF32GM095061
Deutscher Akademischer Austauschdienst (DAAD)UNSPECIFIED
Caltech Summer Undergraduate Research Fellowship (SURF)UNSPECIFIED
FWF Der WissenschaftsfondsJ3327-B21
Subject Keywords:directed evolution; high-throughput screening; protein engineering; terpenes; thermostability
Issue or Number:21
PubMed Central ID:PMC3828198
DOI:10.1002/anie.201301362
Record Number:CaltechAUTHORS:20130701-105908055
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130701-105908055
Official Citation:Lauchli, R., Rabe, K. S., Kalbarczyk, K. Z., Tata, A., Heel, T., Kitto, R. Z. and Arnold, F. H. (2013), High-Throughput Screening for Terpene-Synthase-Cyclization Activity and Directed Evolution of a Terpene Synthase . Angew. Chem. Int. Ed., 52: 5571–5574. doi: 10.1002/anie.201301362
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39162
Collection:CaltechAUTHORS
Deposited By: John Wade
Deposited On:01 Jul 2013 21:12
Last Modified:09 Nov 2021 23:43

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