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Activated GTPase movement on an RNA scaffold drives cotranslational protein targeting

Shen, Kuang and Arslan, Sinan and Akopian, David and Ha, Taekjip and Shan, Shu-ou (2013) Activated GTPase movement on an RNA scaffold drives cotranslational protein targeting. FASEB Journal, 27 . Art. No. 556.3. ISSN 0892-6638. https://resolver.caltech.edu/CaltechAUTHORS:20130711-133423197

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Abstract

Signal Recognition Particle (SRP) and its receptor (SR) are GTPases that translocate ribosome-nascent chain complexes (RNCs) from cytosol to cellular membranes. SRP recognizes the RNC, and through interactions with SR, brings the RNC to the membrane. Subsequent rearrangements activate GTP hydrolysis and load the RNC onto the translocon. During this process, the SRP RNA plays an indispensible role in stimulating both SRP-SR complex formation and the following GTP hydrolysis. Previous studies hinted that the SRP RNA is a bi-functional molecule with two distinct functional ends, the tetraloop end and the distal end, that each stimulates the SRP-SR complex formation and GTP hydrolysis steps. Here we used single molecule technique to directly visualize the global rearrangement of the GTPase complex from the RNA’s tetraloop end to the distal end, traveling over 100 Angstrom in the targeting reaction. Moreover, we showed that the rearrangement is tightly controlled by the RNC and the translocon. This large-scale movement of the GTPase complex provides a molecular mechanism of coupling GTPase activation to the transfer of RNC, thereby ensuring productive protein targeting.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://www.fasebj.org/cgi/content/meeting_abstract/27/1_MeetingAbstracts/556.3PublisherArticle
ORCID:
AuthorORCID
Shan, Shu-ou0000-0002-6526-1733
Additional Information:© 2013 FASEB.
Record Number:CaltechAUTHORS:20130711-133423197
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130711-133423197
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39320
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:12 Aug 2013 22:37
Last Modified:03 Oct 2019 05:06

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