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Biomolecular resource utilization in elementary cell-free gene circuits

Siegal-Gaskins, Dan and Noireaux, Vincent and Murray, Richard M. (2013) Biomolecular resource utilization in elementary cell-free gene circuits. In: 2013 American Control Conference (ACC). IEEE , Piscataway, NJ, pp. 1531-1536. ISBN 978-1-4799-0177-7. http://resolver.caltech.edu/CaltechAUTHORS:20130715-075915746

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Abstract

We present a detailed dynamical model of the behavior of transcription-translation circuits in vitro that makes explicit the roles played by essential molecular resources. A set of simple two-gene test circuits operating in a cell-free biochemical 'breadboard' validate this model and highlight the consequences of limited resource availability. In particular, we are able to confirm the existence of biomolecular 'crosstalk' and isolate its individual sources. The implications of crosstalk for biomolecular circuit design and function are discussed.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://arxiv.org/abs/1307.0178arXivDiscussion Paper
https://doi.org/10.1109/ACC.2013.6580053DOIArticle
ORCID:
AuthorORCID
Murray, Richard M.0000-0002-5785-7481
Additional Information:© 2013 AACC. We thank Z. Sun, C. Hayes, J. Kim, and E. Yeung for help with TX-TL experiments and useful discussions. We also thank J. B. Lucks for the untranslated RNA gene construct. This work is supported by the Institute for Collaborative Biotechnologies through grant W911NF-09-0001 from the U.S. Army Research Office, and the DARPA Living Foundries Program under Contract HR0011-12-C-0065. The content of the information does not necessarily reflect the position or the policy of the Government, and no official endorsement should be inferred.
Funders:
Funding AgencyGrant Number
Army Research Office (ARO)W911NF-09-0001
Defense Advanced Research Projects Agency (DARPA)HR0011-12-C-0065
Record Number:CaltechAUTHORS:20130715-075915746
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20130715-075915746
Official Citation:Siegal-Gaskins, D.; Noireaux, V.; Murray, R.M., "Biomolecular resource utilization in elementary cell-free gene circuits," American Control Conference (ACC), 2013 , vol., no., pp.1531,1536, 17-19 June 2013 URL: http://ieeexplore.ieee.org/stamp/stamp.jsp?tp=&arnumber=6580053&isnumber=6579790
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39352
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:06 Aug 2013 22:35
Last Modified:26 Mar 2019 21:09

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