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The transcription factor chicken Scratch2 is expressed in a subset of early postmitotic neural progenitors

Vieceli, Felipe Monteleone and Simões-Costa, Marcos and Turri, José Antonio and Kanno, Tatiane and Bronner, Marianne E. and Yan, Chao Yun Irene (2013) The transcription factor chicken Scratch2 is expressed in a subset of early postmitotic neural progenitors. Gene Expression Patterns, 13 (5-6). pp. 189-196. ISSN 1567-133X. https://resolver.caltech.edu/CaltechAUTHORS:20130722-140013924

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Abstract

Scratch proteins are members of the Snail superfamily which have been shown to regulate invertebrate neural development. However, in vertebrates, little is known about the function of Scratch or its relationship to other neural transcription factors. We report the cloning of chicken Scratch2 (cScrt2) and describe its expression pattern in the chick embryo from HH15 through HH29. cScrt2 was detected in cranial ganglia, the nasal placode and neural tube. At all stages examined, cScrt2 expression is only detected within a subregion of the intermediate zone of the neural tube. cScrt2 is also expressed in the developing dorsal root ganglia from HH22–23 onwards and becomes limited to its dorsal medial domain at HH29. phospho-Histone H3 and BrdU-labeling revealed that the cScrt2 expression domain is located immediately external to the proliferative region. In contrast, cScrt2 domain overlapped almost completely with that of the postmitotic neural transcription factor NeuroM/Ath3/NEUROD4. Together, these data define cScrt2-positive cells as a subset of immediately postmitotic neural progenitors. Previous data has shown that Scrt2 is a repressor of E-box-driven transcription whereas NeuroM is an E-box-transactivator. In light of these data, the co-localization detected here suggests that Scrt2 and NeuroM may have opposing roles during definition of neural subtypes.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.gep.2013.03.004 DOIArticle
http://www.sciencedirect.com/science/article/pii/S1567133X13000379PublisherArticle
ORCID:
AuthorORCID
Vieceli, Felipe Monteleone0000-0001-5142-8224
Simões-Costa, Marcos0000-0003-1452-7068
Bronner, Marianne E.0000-0003-4274-1862
Yan, Chao Yun Irene0000-0003-0527-5719
Additional Information:© 2013 Elsevier B.V. Received 13 March 2012; Received in revised form 19 March 2013; Accepted 21 March 2013; Available online 6 April 2013. We thank Dr. Shankar Srinivas for sharing the protocol for in situ hybridization in paraffin sections and Dr. Cristóvão de Albuquerque for critically reading this manuscript. This work was supported by a grant from FAPESP (2009/50544-1) and CNPq to CYIY. FMV was supported by a fellowship from FAPESP.
Funders:
Funding AgencyGrant Number
Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)2009/50544-1
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)UNSPECIFIED
Issue or Number:5-6
Record Number:CaltechAUTHORS:20130722-140013924
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130722-140013924
Official Citation:Felipe Monteleone Vieceli, Marcos Simões-Costa, José Antonio Turri, Tatiane Kanno, Marianne Bronner, Chao Yun Irene Yan, The transcription factor chicken Scratch2 is expressed in a subset of early postmitotic neural progenitors, Gene Expression Patterns, Volume 13, Issues 5–6, June–July 2013, Pages 189-196, ISSN 1567-133X, http://dx.doi.org/10.1016/j.gep.2013.03.004.
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39502
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:22 Jul 2013 22:49
Last Modified:09 Mar 2020 13:19

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