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Cell type-specific proteomic profiling in Caenorhabditis elegans

Yuet, Kai P. and Doma, Meenakshi K. and Ngo, John T. and Sternberg, Paul W. and Tirrell, David A. (2013) Cell type-specific proteomic profiling in Caenorhabditis elegans. In: 245th ACS National Meeting & Exposition, Abstracts of Papers, April 7-11, 2013, New Orleans, LA.

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In a complex eukaryote like C. Elegans, cell and tissue heterogeneity restricts the usefulness of large-scale, mass spectrometrybased anal. of an organism's proteome. As worm cell lines are not available and enriching for specific cells or tissues is challenging, researchers cannot systematically identify low abundance proteins expressed in specific cells or tissues from wholeworm lysates. To isolate protein from specific cells, we have engineered C. Elegans phenylalanyl-tRNA synthetases capable of appending azide- or alkynyl-bearing analogs of phenylalanine to tRNA^(Phe). As these analogs are not substrates for any of the wild-type aminoacyl-tRNA synthetases in C. Elegans, we achieved cell type selectivity by spatially restricting the expression of a mutant synthetase using cell type-specific promoters. Because proteins from those cells that express the mutant synthetase - and only those cells - contain the analogs, they can be enriched by treatment with alkyne- or azide-functionalized biotin reagents and subsequent affinity chromatog. for identification by LC/MS-MS.

Item Type:Conference or Workshop Item (Paper)
Related URLs:
URLURL TypeDescription website
Yuet, Kai P.0000-0002-1381-8923
Sternberg, Paul W.0000-0002-7699-0173
Tirrell, David A.0000-0003-3175-4596
Additional Information:© 2013 American Chemical Society.
Record Number:CaltechAUTHORS:20130802-084724182
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39723
Deposited By: Tony Diaz
Deposited On:05 Aug 2013 23:40
Last Modified:03 Oct 2019 05:10

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