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DNA electrochemistry of the E. coli helicase, DinG

Grodick, Michael A. and Barton, Jacqueline K. (2013) DNA electrochemistry of the E. coli helicase, DinG. In: 245th ACS National Meeting & Exposition, Abstracts of Papers, April 7-11, 2013, New Orleans, LA.

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Coli helicase DinG, which can be induced by DNA damage, previously has been shown to have a [4Fe-4S] cluster. While the primary in vivo role of DinG has not been revealed, the protein has been implicated in clearing stalled replication forks that arise from the collision of oppositely oriented transcription and replication machinery. We have explored the DNA-bound redox potential of DinG using electrochem. on gold modified with a helicase substrate, a 20-mer oligonucleotide with a 15-mer overhang. The [4Fe-4S] cluster can be reduced and oxidized reversibly at a DNA-bound redn. potential of ∼ 80 mV vs. This DNA-mediated electrochem. signal, moreover, is stimulated by ATP-hydrolysis. Results from cellular activity assays suggest that DinG may cooperate with other DNA-processing enzymes that have [4Fe-4S] clusters to locate and process DNA damage products.

Item Type:Conference or Workshop Item (Paper)
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Barton, Jacqueline K.0000-0001-9883-1600
Additional Information:© 2013 American Chemical Society.
Record Number:CaltechAUTHORS:20130802-084957563
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39724
Deposited By: Tony Diaz
Deposited On:05 Aug 2013 23:42
Last Modified:03 Oct 2019 05:10

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