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Wide field-of-view Talbot grid-based microscopy for multicolor fluorescence imaging

Pang, Shuo and Han, Chao and Erath, Jessey and Rodriguez, Ana and Yang, Changhuei (2013) Wide field-of-view Talbot grid-based microscopy for multicolor fluorescence imaging. Optics Express, 21 (12). pp. 14555-14565. ISSN 1094-4087. PMCID PMC3726246.

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The capability to perform multicolor, wide field-of-view (FOV) fluorescence microscopy imaging is important in screening and pathology applications. We developed a microscopic slide-imaging system that can achieve multicolor, wide FOV, fluorescence imaging based on the Talbot effect. In this system, a light-spot grid generated by the Talbot effect illuminates the sample. By tilting the excitation beam, the Talbot-focused spot scans across the sample. The images are reconstructed by collecting the fluorescence emissions that correspond to each focused spot with a relay optics arrangement. The prototype system achieved an FOV of 12 × 10 mm^2 at an acquisition time as fast as 23 s for one fluorescence channel. The resolution is fundamentally limited by spot size, with a demonstrated full-width at half-maximum spot diameter of 1.2 μm. The prototype was used to nimage green fluorescent beads, double-stained human breast cancer SK-BR-3 cells, Giardia lamblia cysts, and the Cryptosporidium parvum oocysts. This imaging method is scalable and simple for implementation of high-speed wide FOV fluorescence microscopy.

Item Type:Article
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Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2013 Optical Society of America. Received 14 May 2013; revised 5 Jun 2013; accepted 5 Jun 2013; published 11 Jun 2013. We thank Hao Yuan Kueh (Division of Biology, Caltech) for helpful discussions on large-FOV imaging based on conventional microscopy. This project was funded by the NIH under grant 1R01AI096226-01.
Funding AgencyGrant Number
Issue or Number:12
Classification Code:OCIS codes: (180.2520) Fluorescence microscopy; (110.6760) Talbot and self-imaging effects
PubMed Central ID:PMC3726246
Record Number:CaltechAUTHORS:20130805-103927291
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Official Citation:Optics Express, Vol. 21, Issue 12, pp. 14555-14565 (2013)
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39762
Deposited By: Tony Diaz
Deposited On:05 Aug 2013 23:16
Last Modified:09 Mar 2020 13:19

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