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Wide-field, high-resolution Fourier ptychographic microscopy

Zheng, Guoan and Horstmeyer, Roarke and Yang, Changhuei (2013) Wide-field, high-resolution Fourier ptychographic microscopy. Nature Photonics, 7 (9). pp. 739-745. ISSN 1749-4885. PMCID PMC4169052.

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We report an imaging method, termed Fourier ptychographic microscopy (FPM), which iteratively stitches together a number of variably illuminated, low-resolution intensity images in Fourier space to produce a wide-field, high-resolution complex sample image. By adopting a wavefront correction strategy, the FPM method can also correct for aberrations and digitally extend a microscope’s depth of focus beyond the physical limitations of its optics. As a demonstration, we built a microscope prototype with a resolution of 0.78 µm, a field of view of ∼120 mm^2 and a resolution-invariant depth of focus of 0.3 mm (characterized at 632 nm). Gigapixel colour images of histology slides verify successful FPM operation. The reported imaging procedure transforms the general challenge of high-throughput, high-resolution microscopy from one that is coupled to the physical limitations of the system’s optics to one that is solvable through computation.

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Horstmeyer, Roarke0000-0002-2480-9141
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2013 Macmillan Publishers Limited. Received 27 January 2013; Accepted 21 June 2013; Published online 28 July 2013. The authors thank Xiaoze Ou for discussions and help with experiments. The authors acknowledge funding support from the National Institutes of Health (grant no. 1DP2OD007307-01). Author contributions: G.Z. initiated this line of investigation, designed and implemented the project. G.Z., R.H. and C.Y. contributed, developed, refined the concept and wrote the paper.
Errata:Corrigendum: Wide-field, high-resolution Fourier ptychographic microscopy Guoan Zheng, Roarke Horstmeyer and Changhuei Yang Nature Photonics 7, 739–745 (2013); published online 28 July 2013; corrected after print 30 July 2015
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Issue or Number:9
PubMed Central ID:PMC4169052
Record Number:CaltechAUTHORS:20130808-111715101
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39821
Deposited By: Tony Diaz
Deposited On:08 Aug 2013 21:11
Last Modified:09 Mar 2020 13:19

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