Whited, Charlotte A. and Warren, Jeffrey J. and Lavoie, Katherine D. and Winkler, Jay R. and Gray, Harry B. (2013) Kinetics of CO recombination to the heme in Geobacillus stearothermophilus nitric oxide synthase. Polyhedron, 58 . pp. 134-138. ISSN 0277-5387. PMCID PMC3747573. doi:10.1016/j.poly.2012.08.079. https://resolver.caltech.edu/CaltechAUTHORS:20130815-084001492
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Abstract
We report the kinetics of CO rebinding to the heme in His134Ser, Ile223Val and His134Ser/Ile223Ser mutants of Geobacillus stearothermophilus nitric oxide synthase (gsNOS). The amplitudes of the two observed kinetics phases, which are insensitive to CO concentration, depend on enzyme concentration. We suggest that two forms of gsNOS are in equilibrium under the conditions employed (6.1–27 μM gsNOS with 20 or 100% CO atmosphere). The kinetics of CO rebinding to the heme do not depend on the identity of the NO-gate residues at positions 134 and 223.
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| Additional Information: | © 2012 Elsevier Ltd. Available online 21 September 2012. Our work was supported by NIH (DK019038 to H.B.G. and J.R.W.) and (GM095037 to J.J.W.). | |||||||||
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| Subject Keywords: | Nitric oxide synthase; Carbon monoxide; Heme proteins | |||||||||
| PubMed Central ID: | PMC3747573 | |||||||||
| DOI: | 10.1016/j.poly.2012.08.079 | |||||||||
| Record Number: | CaltechAUTHORS:20130815-084001492 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20130815-084001492 | |||||||||
| Official Citation: | Charlotte A. Whited, Jeffrey J. Warren, Katherine D. Lavoie, Jay R. Winkler, Harry B. Gray, Kinetics of CO recombination to the heme in Geobacillus stearothermophilus nitric oxide synthase, Polyhedron, Volume 58, 13 July 2013, Pages 134-138, ISSN 0277-5387, http://dx.doi.org/10.1016/j.poly.2012.08.079. | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 39936 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Tony Diaz | |||||||||
| Deposited On: | 16 Aug 2013 21:54 | |||||||||
| Last Modified: | 09 Nov 2021 23:48 |
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