Zheng, Guoan and Ou, Xiaoze and Horstmeyer, Roarke and Yang, Changhuei (2013) Characterization of spatially varying aberrations for wide field-of-view microscopy. Optics Express, 21 (13). pp. 15131-15143. ISSN 1094-4087. PMCID PMC3724395. doi:10.1364/OE.21.015131. https://resolver.caltech.edu/CaltechAUTHORS:20130815-084711799
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Abstract
We describe a simple and robust approach for characterizing the spatially varying pupil aberrations of microscopy systems. In our demonstration with a standard microscope, we derive the location-dependent pupil transfer functions by first capturing multiple intensity images at different defocus settings. Next, a generalized pattern search algorithm is applied to recover the complex pupil functions at ~350 different spatial locations over the entire field-of-view. Parameter fitting transforms these pupil functions into accurate 2D aberration maps. We further demonstrate how these aberration maps can be applied in a phase-retrieval based microscopy setup to compensate for spatially varying aberrations and to achieve diffraction-limited performance over the entire field-of-view. We believe that this easy-to-use spatially-varying pupil characterization method may facilitate new optical imaging strategies for a variety of wide field-of-view imaging platforms.
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Additional Information: | © 2013 Optical Society of America. Received 15 May 2013; revised 31 May 2013; accepted 10 Jun 2013; published 17 Jun 2013. We acknowledge funding support from National Institute of Health under Grant No. 1R01AI096226-01. | ||||||||||||
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Issue or Number: | 13 | ||||||||||||
Classification Code: | OCIS codes: (170.0180) Microscopy; (100.0100) Image processing | ||||||||||||
PubMed Central ID: | PMC3724395 | ||||||||||||
DOI: | 10.1364/OE.21.015131 | ||||||||||||
Record Number: | CaltechAUTHORS:20130815-084711799 | ||||||||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20130815-084711799 | ||||||||||||
Official Citation: | Optics Express, Vol. 21, Issue 13, pp. 15131-15143 (2013) http://dx.doi.org/10.1364/OE.21.015131 | ||||||||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||||||||
ID Code: | 39937 | ||||||||||||
Collection: | CaltechAUTHORS | ||||||||||||
Deposited By: | Tony Diaz | ||||||||||||
Deposited On: | 16 Aug 2013 21:22 | ||||||||||||
Last Modified: | 09 Nov 2021 23:48 |
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