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Characterization of spatially varying aberrations for wide field-of-view microscopy

Zheng, Guoan and Ou, Xiaoze and Horstmeyer, Roarke and Yang, Changhuei (2013) Characterization of spatially varying aberrations for wide field-of-view microscopy. Optics Express, 21 (13). pp. 15131-15143. ISSN 1094-4087. PMCID PMC3724395. https://resolver.caltech.edu/CaltechAUTHORS:20130815-084711799

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Abstract

We describe a simple and robust approach for characterizing the spatially varying pupil aberrations of microscopy systems. In our demonstration with a standard microscope, we derive the location-dependent pupil transfer functions by first capturing multiple intensity images at different defocus settings. Next, a generalized pattern search algorithm is applied to recover the complex pupil functions at ~350 different spatial locations over the entire field-of-view. Parameter fitting transforms these pupil functions into accurate 2D aberration maps. We further demonstrate how these aberration maps can be applied in a phase-retrieval based microscopy setup to compensate for spatially varying aberrations and to achieve diffraction-limited performance over the entire field-of-view. We believe that this easy-to-use spatially-varying pupil characterization method may facilitate new optical imaging strategies for a variety of wide field-of-view imaging platforms.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1364/OE.21.015131 DOIArticle
http://www.opticsinfobase.org/oe/abstract.cfm?uri=oe-21-13-15131PublisherArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3724395/PubMed CentralArticle
ORCID:
AuthorORCID
Horstmeyer, Roarke0000-0002-2480-9141
Yang, Changhuei0000-0001-8791-0354
Additional Information:© 2013 Optical Society of America. Received 15 May 2013; revised 31 May 2013; accepted 10 Jun 2013; published 17 Jun 2013. We acknowledge funding support from National Institute of Health under Grant No. 1R01AI096226-01.
Funders:
Funding AgencyGrant Number
NIH1R01AI096226-01
Issue or Number:13
Classification Code:OCIS codes: (170.0180) Microscopy; (100.0100) Image processing
PubMed Central ID:PMC3724395
Record Number:CaltechAUTHORS:20130815-084711799
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130815-084711799
Official Citation:Optics Express, Vol. 21, Issue 13, pp. 15131-15143 (2013) http://dx.doi.org/10.1364/OE.21.015131
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:39937
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:16 Aug 2013 21:22
Last Modified:09 Mar 2020 13:19

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