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The quantitation of fluorescence by photography

Pulleyblank, David E. and Shure, Mavis and Vinograd, Jerome (1977) The quantitation of fluorescence by photography. Nucleic Acids Research, 4 (5). pp. 1409-1418. ISSN 0305-1048. PMCID PMC343764. doi:10.1093/nar/4.5.1409.

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A method based on theory has been developed for the photographic quantitation of fluorescent substances. DNA stained with ethidium in agarose gels is used as an example of an application of this method. In the course of developing this method we have demonstrated that the empirical methods employed by other authors can give rise to large systematic errors. We have also developed an approximate method based on photographic theory, avoiding the use of digital integration which is required by the rigorous method.

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Additional Information:© 1977 Oxford University Press. Received January 1, 1977. We wish to thank Dr. M. Ross and M. Klymkowsky for their help in calibrating the step density wedges. This work was supported in part by USPHS, National Institutes of Health Grants GM15327 and CA 08014. D. E. P. is a recipient of a Medical Research Council of Canada fellowship. M. S. is a recipient of an N. S. F. graduate fellowship. This is contribution No. 5426 from the Division of Chemistry and Chemical Engineering.
Funding AgencyGrant Number
NIHCA 08014
Medical Research Council of CanadaUNSPECIFIED
NSF Graduate Research FellowshipUNSPECIFIED
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Other Numbering System NameOther Numbering System ID
Caltech Division of Chemistry and Chemical Engineering5426
Issue or Number:5
PubMed Central ID:PMC343764
Record Number:CaltechAUTHORS:PULnar77
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Official Citation:David E. Pulleyblank, Mavis Shure, Jerome Vinograd, (deceased), The quantitation of fluorescence by photography, Nucleic Acids Research, Volume 4, Issue 5, 1 June 1977, Pages 1409–1418,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4000
Deposited By: Tony Diaz
Deposited On:24 Jul 2006
Last Modified:08 Nov 2021 20:14

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