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Nucleotide sequences of the 5' termini of øX174 mRNAs synthesized in vitro

Smith, Lloyd H. and Grohmann, Karel and Sinsheimer, R. L. (1974) Nucleotide sequences of the 5' termini of øX174 mRNAs synthesized in vitro. Nucleic Acids Research, 1 (11). pp. 1521-1530. ISSN 0305-1048. PMCID PMC343428.

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When using øX17U RFI DNA as a template, in vitro, E. coli RNA polymerase synthesizes four major purine triphosphate-containing 5' end sequences. RNase A digests of 32P labeled RNA were further digested with spleen exonuclease to remove the bulk of the oligonucleotides with 5' hydroxyls and then chromatographed on DEAE cellulose to resolve the remaining 5' terminal oligonucleotides. By application of standard separation and sequence techniques, the major 5' end sequences were shown to be: pppApUp(Cp), pppApApApUp(Cp), pppApApApApUp(Cp), and pppGpApUp(Gp).

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Additional Information:Copyright © 1974 Oxford University Press. Received August 20, 1974. The authors acknowledge the excellent technical assistance of Charles Romeo. This research was supported by grant number GM 13554 from the National Institutes of Health.
Funding AgencyGrant Number
U.S. Public Health Service (USPHS)GM 13554
Issue or Number:11
PubMed Central ID:PMC343428
Record Number:CaltechAUTHORS:SMInar74
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4012
Deposited By: Tony Diaz
Deposited On:24 Jul 2006
Last Modified:02 Oct 2019 23:09

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