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Exploration of long and short repetitive sequence relationships in the sea urchin genome

Eden, Francine C. and Graham, Dale E. and Davidson, Eric H. and Britten, Roy J. (1977) Exploration of long and short repetitive sequence relationships in the sea urchin genome. Nucleic Acids Research, 4 (5). pp. 1553-1567. ISSN 0305-1048. PMCID PMC343773. doi:10.1093/nar/4.5.1553.

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Long and short repetitive sequences of sea urchin DNA were prepared by reassoci-ation of 2000 nucleotide long fragments to Cot 4 and digestion with the single strand specific nuclease S1. The S1 resistant duplexes were separated into long repetitive and short repetitive fractions on Agarose A50. The extent of shared sequences was studied by reassociating a labeled preparation of short repetitive DNA with an excess of unlabeled long repetitive DNA. Less than 10% of the long repetitive DNA preparation was able to reassociate with the short repetitive DNA. Thus the long and short repetitive elements appear to be principally independent sequence classes in sea urchin DNA. Precisely reassociating repetitive DNA was prepared by four successive steps of reassociation and thermal chromatography on hydroxyapatite. This fraction (3% of the genome) was reassociated by itself or with a great excess of total sea urchin DNA. The thermal stability of the products was identical in both cases (Tm=81°C), indicating that precisely repeated sequences do not have many imprecise copies in sea urchin DNA.

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Additional Information:Copyright © 1977 Oxford University Press. Received January 29, 1977. This research was supported by a grant from the National Science Foundation, BMS 75-07359, and by grants from the National Institutes of Health HD 05753 and GM-20927. FCE was the recipient of a fellowship from the American Cancer Society. DEG was supported by fellowship grants from the Damon Runyon-Walter Winchell Cancer Fund and the Carnegie Institution of Washington.
Funding AgencyGrant Number
NSFBMS 75-07359
NIHHD 05753
American Cancer SocietyUNSPECIFIED
Damon Runyon-Walter Winchell Cancer FundUNSPECIFIED
Carnegie Institution of WashingtonUNSPECIFIED
Issue or Number:5
PubMed Central ID:PMC343773
Record Number:CaltechAUTHORS:EDEnar77
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Official Citation:Francine C. Eden, Dale E. Graham, Eric H. Davidson, Roy J. Britten, Exploration of long and short repetitive sequence relationships in the sea urchin genome, Nucleic Acids Research, Volume 4, Issue 5, 1 June 1977, Pages 1553–1568,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4016
Deposited By: Tony Diaz
Deposited On:24 Jul 2006
Last Modified:08 Nov 2021 20:14

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