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A program for least squares analysis of reassociation and hybridization data

Pearson, W. R. and Davidson, E. H. and Britten, R. J. (1977) A program for least squares analysis of reassociation and hybridization data. Nucleic Acids Research, 4 (6). pp. 1727-1737. ISSN 0305-1048. PMCID PMC342517. doi:10.1093/nar/4.6.1727.

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A computer program is described for the rapid calculation of least squares solutions for data fitted to different functions normally used in reassociation and hybridization kinetic measurements. The equations for the fraction not reacted as a function of Cot follow: First order, exp(–kCot); second order, (1+kCot)–1 ; variable order, (1+kCot)–n; approximate fraction of DNA sequence remaining single stranded, (1+kCot)–44; and a function describing the pairing of tracer when the rate constant for the tracer (k) is distinct from the driver rate constant kd): exp{kL1-(1+kdCot)1–nJ/Lkd(1-n)J}. Several components may be used for most of these functional forms. The standard deviations of the individual parameters at the solutions are calculated.

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Additional Information:© 1977 Oxford University Press. Received December 21, 1976. The program described in this paper is available as a punched card deck from the authors. Two versions are available, one for a DECsystem-10 timesharing computer and a second for an IBM 370 Batch processor. Both programs are written in FORTRAN and can be easily modified for other systems; the two programs differ in minor system dependent features.
Issue or Number:6
PubMed Central ID:PMC342517
Record Number:CaltechAUTHORS:PEAnar77
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Official Citation:W.R. Pearson, E.H. Davidson, R.J. Britten, A program for least squares analysis of reassociation and hybridization data, Nucleic Acids Research, Volume 4, Issue 6, 1 June 1977, Pages 1727–1738,
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4037
Deposited By: Tony Diaz
Deposited On:25 Jul 2006
Last Modified:08 Nov 2021 20:14

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