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Microfluidic cartridges preloaded with nanoliter plugs of reagents: an alternative to 96-well plates for screening

Chen, Delai L. and Ismagilov, Rustem F. (2006) Microfluidic cartridges preloaded with nanoliter plugs of reagents: an alternative to 96-well plates for screening. Current Opinion in Chemical Biology, 10 (3). pp. 226-231. ISSN 1367-5931. http://resolver.caltech.edu/CaltechAUTHORS:20130821-160717350

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Abstract

In traditional screening with 96-well plates, microliters of substrates are consumed for each reaction. Further miniaturization is limited by the special equipment and techniques required to dispense nanoliter volumes of fluid. Plug-based microfluidics confines reagents in nanoliter plugs (droplets surrounded by fluorinated carrier fluid), and uses simple pumps to control the flow of plugs. By using cartridges pre-loaded with nanoliter plugs of reagents, only two pumps and a merging junction are needed to set up a screen. Screening with preloaded cartridges uses only nanoliters of substrate per reaction, and requires no microfabrication. The low cost and simplicity of this method has the potential of replacing 96-well and other multi-well plates, and has been applied to enzymatic assays, protein crystallization and optimization of organic reactions.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1016/j.cbpa.2006.04.004DOIArticle
http://www.sciencedirect.com/science/article/pii/S136759310600055XPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1764868/PubMed CentralArticle
ORCID:
AuthorORCID
Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:Copyright © 2006 Elsevier. Available online 3 May 2006. The work described in this review has been supported by the National Institute for Biomedical Imaging and Bioengineering (R01 EB001903), by the National Institute of General Medical Sciences (R01 GM075827), by the Beckman Young Investigator Program and by the DuPont Young Professor Award. We thank our colleagues in the Ismagilov laboratory who have contributed to the work described here.
Funders:
Funding AgencyGrant Number
NIBIBR01 EB001903
NIH Roadmap for Medical ResearchR01 GM075827
Beckman Young Investigator awardUNSPECIFIED
DuPont Young Professor Grant UNSPECIFIED
Subject Keywords:protein crystallization conditions; multistep synthesis; system; droplets; microarray; volumes; assays; scale; time; flow
Record Number:CaltechAUTHORS:20130821-160717350
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20130821-160717350
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40785
Collection:CaltechAUTHORS
Deposited By: Whitney Barlow
Deposited On:27 Aug 2013 22:54
Last Modified:23 Mar 2015 22:25

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