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Using three-phase flow of immiscible liquids to prevent coalescence of droplets in microfluidic channels: Criteria to identify the third liquid and validation with protein crystallization

Chen, Delai L. and Li, Liang and Reyes, Sebastian and Adamson, David N. and Ismagilov, Rustem F. (2007) Using three-phase flow of immiscible liquids to prevent coalescence of droplets in microfluidic channels: Criteria to identify the third liquid and validation with protein crystallization. Langmuir, 23 (4). pp. 2255-2260. ISSN 0743-7463. https://resolver.caltech.edu/CaltechAUTHORS:20130821-160717523

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Abstract

This manuscript describes the effect of interfacial tensions on three-phase liquid-liquid-liquid flow in microfluidic channels and the use of this flow to prevent microfluidic plugs from coalescing. One problem in using microfluidic plugs as microreactors is the coalescence of adjacent plugs caused by the relative motion of plugs during flow. Here, coalescence of reagent plugs was eliminated by using plugs of a third immiscible liquid as spacers to separate adjacent reagent plugs. This work tested the requirements of interfacial tensions for plugs of a third liquid to be effective spacers. Two candidates satisfying the requirements were identified, and one of these liquids was used in the crystallization of protein human Tdp1 to demonstrate its compatibility with protein crystallization in plugs. This method for identifying immiscible liquids for use as a spacer will also be useful for applications involving manipulation of large arrays of droplets in microfluidic channels.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/la062152zDOIArticle
http://pubs.acs.org/doi/full/10.1021/la062152zPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1986632/PubMed CentralArticle
ORCID:
AuthorORCID
Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:Copyright © 2007 American Chemical Society. Published In Issue: February 13, 2007. Received July 21, 2006. Revised November 6, 2006. This work was supported in part by ATCG3D, which is funded by the National Institute of General Medical Sciences and National Center for Research Resources under the PSI-2 Specialized Center program (U54 GM074961), and by the NSF MRSEC Program under DMR-0213745. Experiments were performed in part at the MRSEC microfluidic facility funded by NSF. S.R. was supported by the Chicago-Chile Materials Collaboration Program. We thank Thomas A. Witten at University of Chicago for suggestions and comments on the manuscript, and thank Doug Davies, Li Zhang, and Alex Burgin at deCODE biostructures for supplying the purified Tdp1 protein and for advice on crystallization experiments.
Funders:
Funding AgencyGrant Number
NIHU54 GM074961
NSFDMR-0213745
Chicago-Chile Materials Collaboration ProgramUNSPECIFIED
Subject Keywords:tyrosyl-dna phosphodiesterase; monodisperse double emulsions; nanoliter plugs; segmented flow; devices; system; surfactants; capillary; particles; scale
Issue or Number:4
Record Number:CaltechAUTHORS:20130821-160717523
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130821-160717523
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40786
Collection:CaltechAUTHORS
Deposited By: Whitney Barlow
Deposited On:27 Aug 2013 21:46
Last Modified:03 Oct 2019 05:42

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