Seed, Brian (1982) Diazotizable arylamine cellulose papers for the coupling and hybridization of nucleic acids. Nucleic Acids Research, 10 (5). pp. 1799-1810. ISSN 0305-1048. https://resolver.caltech.edu/CaltechAUTHORS:SEEnar82
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Abstract
We describe the synthesis of a family of arylamine-substituted papers which can be diazotized and coupled with nucleic acids. The synthesis is simple and uses readily obtainable starting materials. A partial characterization of the nucleic acid binding activity is reported, as well as a demonstration of the utility of the activated paper for the detection of electrophoretically separated RNA by blot transfer and hybridization.
Item Type: | Article | ||||||
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Additional Information: | Copyright © 1982 Oxford University Press. Received November 10, 1981. Revised February 5, 1982. Accepted February 5, 1982. I would like to thank the many people who shared their experiences with me, traumatic and otherwise; and among them particularly Jim Casey, Chien Yueh-Hsieh, David Goldberg, Ross Hardison, and Liz Lacy. I would also like to thank Tom Maniatis for laboratory space, and my parents for support. | ||||||
Issue or Number: | 5 | ||||||
Record Number: | CaltechAUTHORS:SEEnar82 | ||||||
Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:SEEnar82 | ||||||
Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | ||||||
ID Code: | 4079 | ||||||
Collection: | CaltechAUTHORS | ||||||
Deposited By: | Tony Diaz | ||||||
Deposited On: | 26 Jul 2006 | ||||||
Last Modified: | 02 Oct 2019 23:09 |
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