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Microfluidic arrays of fluid-fluid diffusional contacts as detection elements and combinatorial tools

Ismagilov, Rustem F. and Ng, Jessamine M. K. and Kenis, Paul J. A. and Whitesides, George M. (2001) Microfluidic arrays of fluid-fluid diffusional contacts as detection elements and combinatorial tools. Analytical Chemistry, 73 (21). pp. 5207-5213. ISSN 0003-2700. doi:10.1021/ac010502a.

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This paper describes microfluidic systems that can be used to investigate multiple chemical or biochemical interactions in a parallel format. These three-dimensional systems are generated by crossing two sets of microfluidic channels, fabricated in two different layers, at tight angles. Solutions of the reagents are placed in the channels; in different modes of operation, these solutions can be either flowing or stationary- the latter is important when one set of channels is filled with viscous gels with immobilized reagents. At every crossing, the channels are separated either by a single membrane or by a composite separator comprising a membrane, a microwell, and a second membrane. These components allow diffusive mass transport and minimize convective transport through the crossing. Polycarbonate membranes with 0.1-1-mum vertical pores were used to fabricate the devices. Each crossing of parallel channels serves as an element in which chemical or biochemical interactions can take place; interactions can be detected by monitoring changes in fluorescence and absorbance. These all- organic systems are straightforward to fabricate and to operate and may find applications as portable microanalytical systems and as tools in combinatorial research.

Item Type:Article
Related URLs:
URLURL TypeDescription
Ismagilov, Rustem F.0000-0002-3680-4399
Whitesides, George M.0000-0001-9451-2442
Additional Information:Copyright © 2001 American Chemical Society. Published In Issue: November 01, 2001. Received for review May 2, 2001. Accepted July 24, 2001. This work was supported by DARPA and NSF (ECS-0004030). We thank Steve Metallo (Harvard University) for helpful discussions.
Funding AgencyGrant Number
Subject Keywords:western blots; proteins; systems; fabrication; cells; flows; pdms
Issue or Number:21
Record Number:CaltechAUTHORS:20130821-160719890
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40800
Deposited By: Whitney Barlow
Deposited On:23 Aug 2013 21:20
Last Modified:10 Nov 2021 00:08

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