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Characterization of the Threshold Response of Initiation of Blood Clotting to Stimulus Patch Size

Kastrup, Christian J. and Shen, Feng and Runyon, Matthew K. and Ismagilov, Rustem F. (2007) Characterization of the Threshold Response of Initiation of Blood Clotting to Stimulus Patch Size. Biophysical Journal, 93 (8). pp. 2969-2977. ISSN 0006-3495. doi:10.1529/biophysj.107.109009.

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This article demonstrates that the threshold response of initiation of blood clotting to the size of a patch of stimulus is a robust phenomenon under a wide range of conditions and follows a simple scaling relationship based on the Damkohler number. Human blood and plasma were exposed to surfaces patterned with patches presenting clotting stimuli using microfluidics. Perturbations of the complex network of hemostasis, including temperature, variations in the concentration of stimulus (tissue factor), and the absence or inhibition of individual components of the network (factor IIa, factor V, factor VIII, and thrombomodulin), did not affect the existence of this response. A scaling relationship between the threshold patch size and the timescale of reaction for clotting was supported in numerical simulations, a simple chemical model system, and experiments with human blood plasma. These results may be useful for understanding the spatiotemporal dynamics of other autocatalytic systems and emphasize the relevance of clustering of proteins and lipids in the regulation of signaling processes.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Shen, Feng0000-0002-4709-330X
Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:Copyright © 2007 The Biophysical Society. Received 29 March 2007. Accepted 12 June 2007. Available online 7 January 2009. We thank Thuong Van Ha for helpful discussions, Pamela Haltek and Sharice Davis for collecting blood samples, and Jessica M. Price for contributions in editing and writing this article. This work was supported in part by National Science Foundation CAREER Award No. CHE-0349034 and Office of Naval Research grant No. N000140610630. R.F.I. is a Cottrell Scholar of Research Corporation and an A.P. Sloan Research Fellow. Some of this work was performed at the Materials Research Science and Engineering Center microfluidic facility (funded by the National Science Foundation).
Funding AgencyGrant Number
Cottrell Scholar of Research CorporationUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
Issue or Number:8
Record Number:CaltechAUTHORS:20130821-160721703
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40810
Deposited By: Whitney Barlow
Deposited On:27 Aug 2013 22:16
Last Modified:10 Nov 2021 00:08

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