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ABO, D Blood Typing and Subtyping Using Plug-Based Microfluidics

Kline, Timothy R. and Runyon, Matthew K. and Pothiawala, Mohammad and Ismagilov, Rustem F. (2008) ABO, D Blood Typing and Subtyping Using Plug-Based Microfluidics. Analytical Chemistry, 80 (16). pp. 6190-6197. ISSN 0003-2700. doi:10.1021/ac800485q. https://resolver.caltech.edu/CaltechAUTHORS:20130821-160722469

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Abstract

A plug-based microfluidic approach was used to perform multiple agglutination assays in parallel without crosscontamination and using only microliter volumes of blood. To perform agglutination assays on-chip, a microfluidic device was designed to combine aqueous streams of antibody, buffer, and red blood cells (RBCs) to form droplets 30-40 nL in volume surrounded by a fluorinated carrier fluid. Using this approach, proof-of-concept ABO and D (Rh) blood typing and group A subtyping were successfully performed by screening against multiple antigens without cross-contamination. On-chip subtyping distinguished common A1 and A2 RBCs by using a lectinbased dilution assay. This flexible platform was extended to differentiate rare, weakly agglutinating RBCs of A subtypes by analyzing agglutination avidity as a function of shear rate. Quantitative analysis of changes in contrast within plugs revealed subtleties in agglutination kinetics and enabled characterization of agglutination of rare blood subtypes. Finally, this platform was used to detect bacteria, demonstrating the potential usefulness of this assay in detecting sepsis and the potential for applications in agglutination-based viral detection. The speed, control, and minimal sample consumption provided by this technology present an advance for point of care applications, blood typing of newborns, and general blood assays in small model organisms.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1021/ac800485qDOIArticle
http://pubs.acs.org/doi/full/10.1021/ac800485qPublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2592685/PubMed CentralArticle
ORCID:
AuthorORCID
Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:Copyright © 2008 American Chemical Society. Published In Issue: August 15, 2008. Article ASAP: July 23, 2008. Received: March 07, 2008. Accepted: April 24, 2008. This work was supported in part by the NIH and the NIBIB (Grant R01 EB001903) the Camille Dreyfus Teacher-Scholar Award. M.K.R. was supported in part by Burroughs Wellcome Fund Interfaces I.D. 1001447. R.F.I. is a Cottrell Scholar of the Research Corporation and an A. P. Sloan Research Fellow. Some of this work was performed at the MRSEC microfluidics facility (funded by the NSF). We thank Rebecca R. Pompano for helpful discussions and Jessica M. Price for assistance in writing and editing the manuscript.
Funders:
Funding AgencyGrant Number
NIHR01 EB001903
Camille and Henry Dreyfus FoundationUNSPECIFIED
Burroughs Wellcome Fund Interfaces1001447
Cottrell Scholar of Research CorporationUNSPECIFIED
Alfred P. Sloan FoundationUNSPECIFIED
NSFUNSPECIFIED
Issue or Number:16
DOI:10.1021/ac800485q
Record Number:CaltechAUTHORS:20130821-160722469
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130821-160722469
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40815
Collection:CaltechAUTHORS
Deposited By: Whitney Barlow
Deposited On:27 Aug 2013 20:20
Last Modified:10 Nov 2021 00:09

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