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Protein Crystallization using Microfluidic Technologies Based on Valves, Droplets, and SlipChip

Li, Liang and Ismagilov, Rustem F. (2010) Protein Crystallization using Microfluidic Technologies Based on Valves, Droplets, and SlipChip. Annual Review of Biophysics, 39 . pp. 139-158. ISSN 1936-122X.

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To obtain protein crystals, researchers must search for conditions in multidimensional chemical space. Empirically, thousands of crystallization experiments are carried out to screen various precipitants at multiple concentrations. Microfluidics can manipulate fluids on a nanoliter scale, and it affects crystallization twofold. First, it miniaturizes the experiments that can currently be done on a larger scale and enables crystallization of proteins that are available only in small amounts. Second, it offers unique experimental approaches that are difficult or impossible to implement on a larger scale. Ongoing development of microfluidic techniques and their integration with protein production, characterization, and in situ diffraction promises to accelerate the progress of structural biology.

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Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:Copyright �c 2010 by Annual Reviews. First published online as a Review in Advance on February 1, 2010. DISCLOSURE STATEMENT: The authors are listed as coinventors on University of Chicago patents or patent applications for some methods presented in this article. Rustem F. Ismagilov was a consultant to deCODE Biostructures. ACKNOWLEDGMENTS: Authors work in this area was supported by the Department of Energy under section H.35 of Department of Energy Contract No. DE-AC02–06CH11357 to UChicago Argonne, LLC, to manage Argonne National Laboratory, NIH Protein Structure Initiative Specialized Centers Grant GM074961 (ATCG3D), and the National Institutes of Health through the NIH Roadmap for Medical Research, Grant 5 R01 GM075827. Information on Membrane Protein Production and Structure Determination can be found at We thank Elizabeth B. Haney and Heidi Park for their contributions to writing and editing this manuscript.
Funding AgencyGrant Number
Department of Energy (DOE)DE-AC02–06CH11357
NIH Roadmap for Medical Research5 R01 GM075827
Subject Keywords:free interface diffusion, high throughput, screening, phase diagram, in situ diffraction
Record Number:CaltechAUTHORS:20130821-160723789
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40823
Deposited By: Whitney Barlow
Deposited On:24 Aug 2013 00:00
Last Modified:30 Mar 2015 22:37

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