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Attachment of cells to islands presenting gradients of adhesion ligands

Petty, Rafe T. and Li, Hung-Wing and Maduram, Jane H. and Ismagilov, Rustem and Mrksich, Milan (2007) Attachment of cells to islands presenting gradients of adhesion ligands. Journal of the American Chemical Society, 129 (29). pp. 8966-8967. ISSN 0002-7863. doi:10.1021/ja0735709.

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This paper reports a strategy that uses microfluidic networks to pattern self-assembled monolayers with gradient microislands for the attachment of individual cells. A microfluidic network is first used to pattern a monolayer into square regions that present maleimide groups and then used to flow a solution having a gradient of the cell adhesion peptide Arg-Gly-Asp over the substrate. In this way, the surface is patterned with microislands approximately 33 x 33 micrometers in size and each having a defined gradient of immobilized cell adhesion ligand. B16F10 cells were allowed to attach to the patterned islands and were found to display a nonuniform distribution of cytoskeletal structures in response to the gradient of adhesion ligand. This work is significant because it permits studies of the influence of a nonuniform microenvironment on the polarization, differentiation, and signaling of adherent cells.

Item Type:Article
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URLURL TypeDescription CentralArticle Information
Ismagilov, Rustem0000-0002-3680-4399
Additional Information:Copyright © 2007 American Chemical Society. Published In Issue: July 25, 2007. Received May 18, 2007. This work was supported by NSF-MRSEC and NIH. R.T.P. was supported by the NIH Chemistry & Biology Interface Pre-Doctoral Training Program (Grant T32 GM008720). Supporting Information Available Details for fabrication of μFNs, preparation of SAMs, cell culture, mass spectrometric analysis, fluorescent images of the gradient, and data analysis. This material is available free of charge via the Internet at
Funding AgencyGrant Number
NIHT32 GM008720
Subject Keywords:self-assembled monolayers; fibronectin; chemotaxis; migration; surfaces
Issue or Number:29
Record Number:CaltechAUTHORS:20130821-160728859
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40853
Deposited By: Whitney Barlow
Deposited On:27 Aug 2013 22:31
Last Modified:10 Nov 2021 00:09

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