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An open reading frame upstream from the nifH gene of Klebsiella pneumoniae

Shen, San-chiun and Xue, Zhong-tian and Kong, Qiu-tong and Wu, Qi-long (1983) An open reading frame upstream from the nifH gene of Klebsiella pneumoniae. Nucleic Acids Research, 11 (12). pp. 4241-4250. ISSN 0305-1048. PMCID PMC326038.

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An open reading frame upstream from nifHDK operon of Klebsiella pneumoniae had been described. The orientation of this open reading frame is opposite to that of nifHDK and sequence homology was found between the open reading frame promoter and the promoter of nifHDK operon. A recombinant plasmid carrying the promoter region of the open reading frame fused to the beta-galactosidase gene was constructed. Strains of E.coli were transformed with the plasmid containing this open reading frame promoter-lacZ fusion or co-transformed with it and a plasmid carrying the nifA gene. An appreciable activity of beta-galactosidase was found in strains which received both plasmids, indicating that the promoter of the open reading frame can be activated by the product of nifA gene. Thus, the open reading frame found between nifHDK operon and nifJ behaves just like other nif genes of K.pneumoniae in requiring the product of nifA as the positive effector for expression.

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Additional Information:© 1983 IRL Press Limited, Oxford, England. Received 21 March 1983; Revised 13 May 1983; Accepted 19 May 1983. We would like to thank Dr. F. Ausubel for kindly providing us the pMC1 plasmid, unpublished nifH sequence data and for information concerning the in vitro transcription of a DNA sequence upstream from nifH. We are also grateful to Dr. J. Bonner and Dr. E. M. Meyerowitz for their valuable suggestions. S S-C and X Z-T were supported by E.S. Gosney fund.
Issue or Number:12
PubMed Central ID:PMC326038
Record Number:CaltechAUTHORS:SHEnar83
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:4086
Deposited By: Tony Diaz
Deposited On:26 Jul 2006
Last Modified:02 Oct 2019 23:09

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