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Patterning cells and their environments using multiple laminar fluid flows in capillary networks

Takayama, Shuichi and McDonald, J. Cooper and Ostuni, Emanuele and Liang, Michael N. and Kenis, Paul J. A. and Ismagilov, Rustem F. and Whitesides, George M. (1999) Patterning cells and their environments using multiple laminar fluid flows in capillary networks. Proceedings of the National Academy of Sciences of the United States of America, 96 (10). pp. 5545-5548. ISSN 0027-8424. PMCID PMC21896. https://resolver.caltech.edu/CaltechAUTHORS:20130821-160733567

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Abstract

This paper describes the use of laminar flow of liquids in capillary systems to pattern the cell culture substrate, to perform patterned cell deposition, and to pattern the cell culture media. We demonstrate the patterning of the cell culture substrate with different proteins, the patterning of different types of cells adjacent to each other, the patterned delivery of chemicals to adhered cells, and performing enzymatic reactions over select cells or over a portion of a cell. This method offers a way to simultaneously control the characteristics of the surface to which cells are attached, the type of cells that are in their vicinity, and the kind of media that cells or part of a cell are exposed to. The method is experimentally simple, highly adaptable, and requires no special equipment except for an elastomeric relief that can be readily prepared by rapid prototyping.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.96.10.5545 DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC21896/PubMed CentralArticle
ORCID:
AuthorORCID
Ismagilov, Rustem F.0000-0002-3680-4399
Whitesides, George M.0000-0001-9451-2442
Additional Information:© 1999, The National Academy of Sciences. Accepted March 17, 1999. Supported by the Defense Advanced Research Planning Agency (DARPA)/Space and Naval Warfare Systems Command, DARPA/Office of Naval Research, the National Science Foundation (NSF ECS-9729405), and the National Institutes of Health (NIH GM30367). We thank Prof. D. E. Ingber (Harvard Medical School) for use of his facilities and Dr. S. Warren (Massachusetts General Hospital) for the gift of E. coli (RB 128). S. T. is a Leukemia Society of America Fellow and thanks the society for a postdoctoral fellowship. J. C. M. thanks the National Science Foundation for a predoctoral fellowship. P. J. A. K. thanks the Netherlands Organization for Scientific Research (NWO) for a postdoctoral fellowship.
Funders:
Funding AgencyGrant Number
Defense Advanced Research Planning Agency (DARPA)UNSPECIFIED
Office of Naval Research (ONR)UNSPECIFIED
NSFECS-9729405
NIHGM30367
Leukemia Society of AmericaUNSPECIFIED
Nederlandse Organisatie voor Wetenschappelijk Onderzoek (NWO)UNSPECIFIED
Subject Keywords:fibronectin; proteins; surfaces; growth; matrix; shape
Issue or Number:10
PubMed Central ID:PMC21896
Record Number:CaltechAUTHORS:20130821-160733567
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130821-160733567
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40879
Collection:CaltechAUTHORS
Deposited By: Whitney Barlow
Deposited On:28 Aug 2013 23:15
Last Modified:04 Jun 2020 22:06

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