A Caltech Library Service

Effects of viscosity on droplet formation and mixing in microfluidic channels

Tice, J. D. and Lyon, A. D. and Ismagilov, R. F. (2004) Effects of viscosity on droplet formation and mixing in microfluidic channels. Analytica Chimica Acta, 507 (1). pp. 73-77. ISSN 0003-2670. doi:10.1016/j.aca.2003.11.024.

Full text is not posted in this repository. Consult Related URLs below.

Use this Persistent URL to link to this item:


This paper characterizes the conditions required to form nanoliter-sized droplets (plugs) of viscous aqueous reagents in flows of inuniscible carrier fluid within microfluidic channels. For both non-viscous (viscosity of 2.0 mPa s) and viscous (viscosity of 18 mPa s) aqueous solutions, plugs formed reliably in a flow of water-immiscible carrier fluid for Capillary number less than 0.01, although plugs were able to form at higher Capillary numbers at lower ratios of the aqueous phase flow rate to the flow rate of the carrier fluid (in all the experiments performed, the Reynolds number was less than 1). The paper also shows that combining viscous and non-viscous reagents can enhance mixing in droplets moving through straight microchannels by providing a nearly ideal initial distribution of reagents within each droplet. The study should facilitate the use of this droplet-based microfluidic platform for investigation of protein crystallization, kinetics, and assays.

Item Type:Article
Related URLs:
URLURL TypeDescription
Ismagilov, R. F.0000-0002-3680-4399
Additional Information:Copyright © 2003 Elsevier. Received 4 August 2003. Revised 22 October 2003. Accepted 7 November 2003. Available online 8 January 2004. This work was supported by the Camille and Henry Dreyfus New Faculty Award, Chicago MRSEC funded by NSF, the ONR Young Investigator Award N00014-03-10482, the NIH (R01 EB001903), and by the Beckman Foundation. The work was performed at the Chicago MRSEC microfluidic facility. We thank H. Song and C.J. Gerdts for performing photolithography at MAL of UIC and for advice. We thank M.R. Bringer for helpful discussions.
Funding AgencyGrant Number
Camille and Henry Dreyfus FoundationUNSPECIFIED
NIBIBR01 EB001903
Arnold and Mabel Beckman FoundationUNSPECIFIED
Subject Keywords:viscosity, microfluidic, mixing, multiphase, droplet, plug total analysis systems; capillary tubes; surface-tension; image-analysis; bubbles; pendant; motion; device
Issue or Number:1
Record Number:CaltechAUTHORS:20130821-160733899
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40881
Deposited By: Whitney Barlow
Deposited On:28 Aug 2013 20:18
Last Modified:10 Nov 2021 04:23

Repository Staff Only: item control page