CaltechAUTHORS
  A Caltech Library Service

Microfluidic stochastic confinement enhances analysis of rare cells by isolating cells and creating high density environments for control of diffusible signals

Vincent, Meghan E. and Liu, Weishan and Haney, Elizabeth B. and Ismagilov, Rustem F. (2010) Microfluidic stochastic confinement enhances analysis of rare cells by isolating cells and creating high density environments for control of diffusible signals. Chemical Society Reviews, 39 (3). pp. 974-984. ISSN 0306-0012. PMCID PMC2829723. https://resolver.caltech.edu/CaltechAUTHORS:20130821-160734997

[img]
Preview
PDF - Published Version
See Usage Policy.

2475Kb
[img]
Preview
PDF - Accepted Version
See Usage Policy.

1338Kb

Use this Persistent URL to link to this item: https://resolver.caltech.edu/CaltechAUTHORS:20130821-160734997

Abstract

Rare cells can be difficult to analyze because they either occur in low numbers or coexist with a more abundant cell type, yet their detection is crucial for diagnosing disease and maintaining human health. In this tutorial review, we introduce the concept of microfluidic stochastic confinement for use in detection and analysis of rare cells. Stochastic confinement provides two advantages: (1) it separates rare single cells from the bulk mixture and (2) it allows signals to locally accumulate to a higher concentration around a single cell than in the bulk mixture. Microfluidics is an attractive method for implementing stochastic confinement because it provides simple handling of small volumes. We present technologies for microfluidic stochastic confinement that utilize both wells and droplets for the detection and analysis of single cells. We address how these microfluidic technologies have been used to observe new behavior, increase speed of detection, and enhance cultivation of rare cells. We discuss potential applications of microfluidic stochastic confinement to fields such as human diagnostics and environmental testing.


Item Type:Article
Related URLs:
URLURL TypeDescription
https://doi.org/10.1039/B917851ADOIArticle
https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2829723PubMed CentralArticle
ORCID:
AuthorORCID
Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:© Royal Society of Chemistry 2010. Received 1st September 2009. First published on the web 12th January 2010. This work was supported by ONR Grant N000140610630, NSF CRC Grant No. 0526693 and NIH Director’s Pioneer Award DP1 OD003584 to R.F.I. We thank Rebecca Pompano, James Boedicker, and Elena Lucchetta for helpful discussion and Heidi Park for contributions to writing and editing this manuscript. Part of the themed issue: From microfluidic application to nanofluidic phenomena.
Funders:
Funding AgencyGrant Number
Office of Naval Research (ONR)N000140610630
NSFCHE-0526693
NIH1DP1 OD003584
Issue or Number:3
PubMed Central ID:PMC2829723
Record Number:CaltechAUTHORS:20130821-160734997
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130821-160734997
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40888
Collection:CaltechAUTHORS
Deposited By: Whitney Barlow
Deposited On:26 Aug 2013 22:14
Last Modified:05 Mar 2020 18:09

Repository Staff Only: item control page