Zheng, Bo and Ismagilov, Rustem F. (2005) A Microfluidic Approach for Screening Submicroliter Volumes against Multiple Reagents by Using Preformed Arrays of Nanoliter Plugs in a Three-Phase Liquid/Liquid/Gas Flow. Angewandte Chemie International Edition, 44 (17). pp. 2520-2523. ISSN 1433-7851. doi:10.1002/anie.200462857. https://resolver.caltech.edu/CaltechAUTHORS:20130821-160735736
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Abstract
Plugging a gap in screening: Arrays of nanoliter-sized plugs of different compositions can be preformed in a three-phase liquid/liquid/gas flow. The arrays can be transported into a microfluidic channel to test against a target (see schematic representation), as demonstrated in protein crystallization and an enzymatic assay.
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| Additional Information: | Copyright © 2005 WILEY-VCH. Issue published online: 21 APR 2005. Article first published online: 22 MAR 2005. Manuscript Received: 9 DEC 2004. This work was supported by the National Institutes of Health (grant no. R01 EB001903) and by the Beckman Young Investigator Program and was performed at the MRSEC microfluidic facility funded by the National Science Foundation. We thank David Adamson for invaluable experimental assistance. | |||||||||||||||
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| Subject Keywords: | crystal growth; enzymatic arrays; microreactors; screening methods; three-phase system protein crystallization conditions; chip; system; droplets; device; chemistry; diffusion; transport | |||||||||||||||
| Issue or Number: | 17 | |||||||||||||||
| DOI: | 10.1002/anie.200462857 | |||||||||||||||
| Record Number: | CaltechAUTHORS:20130821-160735736 | |||||||||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20130821-160735736 | |||||||||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||||||||
| ID Code: | 40892 | |||||||||||||||
| Collection: | CaltechAUTHORS | |||||||||||||||
| Deposited By: | Whitney Barlow | |||||||||||||||
| Deposited On: | 28 Aug 2013 19:44 | |||||||||||||||
| Last Modified: | 10 Nov 2021 04:23 |
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