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Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets

Zheng, Bo and Roach, L. Spencer and Ismagilov, Rustem F. (2003) Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets. Journal of the American Chemical Society, 125 (37). pp. 11170-11171. ISSN 0002-7863. doi:10.1021/ja037166v.

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Protein crystallization is a major bottleneck in determining tertiary protein structures from genomic sequence data. This paper describes a microfluidic system for screening hundreds of protein crystallization conditions using less than 4 nL of protein solution for each crystallization droplet. The droplets are formed by mixing protein, precipitant, and additive stock solutions in variable ratios in a flow of water-immiscible fluids inside microchannels. Each droplet represents a discrete trial testing different conditions. The system has been validated by crystallization of several water-soluble proteins.

Item Type:Article
Related URLs:
URLURL TypeDescription
Zheng, Bo0000-0001-8344-3445
Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:Copyright © 2003 American Chemical Society. Published In Issue: September 17, 2003. Received July 9, 2003. This work was supported by the Searle Scholars Program and by the Beckman Young Investigator Program, and was performed at the MRSEC microfluidic facility funded by NSF. We thank Professors Keith Moffat, Phoebe Rice, and Chuan He for valuable suggestions, and we thank H. Song and J. Tice for experimental advice.
Funding AgencyGrant Number
Searle Scholars ProgramUNSPECIFIED
Beckman Young Investigator awardUNSPECIFIED
Subject Keywords:macromolecular crystallization; structural genomics; growth
Issue or Number:37
Record Number:CaltechAUTHORS:20130821-160735877
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40893
Deposited By: Whitney Barlow
Deposited On:28 Aug 2013 21:51
Last Modified:10 Nov 2021 04:23

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