Zheng, Bo and Roach, L. Spencer and Ismagilov, Rustem F. (2003) Screening of protein crystallization conditions on a microfluidic chip using nanoliter-size droplets. Journal of the American Chemical Society, 125 (37). pp. 11170-11171. ISSN 0002-7863. doi:10.1021/ja037166v. https://resolver.caltech.edu/CaltechAUTHORS:20130821-160735877
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Abstract
Protein crystallization is a major bottleneck in determining tertiary protein structures from genomic sequence data. This paper describes a microfluidic system for screening hundreds of protein crystallization conditions using less than 4 nL of protein solution for each crystallization droplet. The droplets are formed by mixing protein, precipitant, and additive stock solutions in variable ratios in a flow of water-immiscible fluids inside microchannels. Each droplet represents a discrete trial testing different conditions. The system has been validated by crystallization of several water-soluble proteins.
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| Additional Information: | Copyright © 2003 American Chemical Society. Published In Issue: September 17, 2003. Received July 9, 2003. This work was supported by the Searle Scholars Program and by the Beckman Young Investigator Program, and was performed at the MRSEC microfluidic facility funded by NSF. We thank Professors Keith Moffat, Phoebe Rice, and Chuan He for valuable suggestions, and we thank H. Song and J. Tice for experimental advice. | |||||||||
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| Subject Keywords: | macromolecular crystallization; structural genomics; growth | |||||||||
| Issue or Number: | 37 | |||||||||
| DOI: | 10.1021/ja037166v | |||||||||
| Record Number: | CaltechAUTHORS:20130821-160735877 | |||||||||
| Persistent URL: | https://resolver.caltech.edu/CaltechAUTHORS:20130821-160735877 | |||||||||
| Usage Policy: | No commercial reproduction, distribution, display or performance rights in this work are provided. | |||||||||
| ID Code: | 40893 | |||||||||
| Collection: | CaltechAUTHORS | |||||||||
| Deposited By: | Whitney Barlow | |||||||||
| Deposited On: | 28 Aug 2013 21:51 | |||||||||
| Last Modified: | 10 Nov 2021 04:23 |
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