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Formation of arrayed droplets of soft lithography and two-phase fluid flow, and application in protein crystallization

Zheng, Bo and Tice, Joshua D. and Ismagilov, Rustem F. (2004) Formation of arrayed droplets of soft lithography and two-phase fluid flow, and application in protein crystallization. Advanced Materials, 16 (15). pp. 1365-1368. ISSN 0935-9648. doi:10.1002/adma.200400590.

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This paper presents an overview of our recent work on the use of soft lithography and two-phase fluid flow to form arrays of droplets. The crucial issues in the formation of stable arrays of droplets and alternating droplets of two sets of aqueous solutions include the geometry of the microchannels, the capillary number, and the water fraction of the system. Glass capillaries could be coupled to the PDMS microchannels and droplets could be transferred into glass capillaries for long-term storage. The arrays of droplets have been applied to screen the conditions for protein crystallization with microbatch and vapor diffusion techniques.

Item Type:Article
Related URLs:
URLURL TypeDescription CentralArticle
Zheng, Bo0000-0001-8344-3445
Ismagilov, Rustem F.0000-0002-3680-4399
Additional Information:Copyright © 2004 WILEY-VCH. Issue published online: 25 AUG 2004. Article first published online: 25 AUG 2004. This work was supported in part by the NIH (R01 EB001903) and the Beckman Young Investigator Program, and was performed at the MRSEC microfluidic facility funded by the NSF.
Funding AgencyGrant Number
NIBIBR01 EB001903
Beckman Young Investigator awardUNSPECIFIED
Subject Keywords:microfluidic devices; reduction photolithography; laminar-flow; poly(dimethylsiloxane); microchannels; fabrication; generation; diffusion; capillaries; systems
Issue or Number:15
Record Number:CaltechAUTHORS:20130821-160736036
Persistent URL:
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40894
Deposited By: Whitney Barlow
Deposited On:28 Aug 2013 20:24
Last Modified:10 Nov 2021 04:23

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