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Corneal Plasticity: Characterization of the Multipotentiality of Human Keratocytes

Chao, J. R. and Bronner-Fraser, M. and Lwigale, P. Y. (2008) Corneal Plasticity: Characterization of the Multipotentiality of Human Keratocytes. ARVO Spring Meeting, 49 . p. 4812. ISSN 0273-0189.

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Purpose: To determine the cell properties of adult human corneal keratocytes when challenged in the chick embryonic environment. Methods: Cultured human keratocytes were injected along cranial neural crest migratory pathways in chick embryos. Human keratocytes were also cultured under various conditions and differentiated into either fibroblasts or myofibroblasts, then transplanted into the chick embryo. Migration of the injected cells was determined by immunohistochemistry using human cell-specific markers and markers of crest derivatives. Results: Injected human keratocytes proliferated and migrated ventrally adjacent to host neural crest cells. They contributed to numerous neural crest-derived tissues including cranial blood vessels, ocular tissues, musculature of the mandibular process, and cardiac cushion tissue. Conclusions: Adult human corneal keratocytes that have undergone terminal differentiation can be induced to form cranial neural crest derivatives when grafted into an embryonic environment.

Item Type:Article
Bronner-Fraser, M.0000-0003-4274-1862
Additional Information:© 2008 by the Association for Research in Vision and Ophthalmology, Inc. Support: Fight for Sight Postdoctoral Fellowship Grant and Heed Fellowship to JRC, NIH grants to MBF, K99/R00 EY018050 to PYL.
Funding AgencyGrant Number
Fight for SightUNSPECIFIED
Heed FellowshipUNSPECIFIED
NIHK99/R00 EY018050
Record Number:CaltechAUTHORS:20130822-083507699
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Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:40904
Deposited By: Tony Diaz
Deposited On:29 Aug 2013 23:33
Last Modified:03 Oct 2019 05:44

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