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Two-photon microscopy of the mouse cochlea in situ for cellular diagnosis

Yang, Xing and Pu, Ye and Hsieh, Chia-Lung and Ong, Cheng Ai and Psaltis, Demetri and Stankovic, Konstantina M. (2013) Two-photon microscopy of the mouse cochlea in situ for cellular diagnosis. Journal of Biomedical Optics, 18 (3). Art. No. 031104 . ISSN 1083-3668. https://resolver.caltech.edu/CaltechAUTHORS:20130904-102718969

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Abstract

Sensorineural hearing loss is the most common type of hearing loss worldwide, yet the underlying cause is typically unknown because the inner ear cannot be biopsied today without destroying hearing, and intracochlear cells have not been imaged with resolution sufficient to establish diagnosis. Intracochlear imaging has been technologically challenging because of the cochlea’s small size and encasement in bone. We report, for the first time, imaging of the mouse cochlea in situ without exogenous dyes, through a membranous round window, using a near-infrared femtosecond laser as the excitation and endogenous two-photon excitation fluorescence (TPEF) and second harmonic generation as the contrast mechanisms. We find that TPEF exhibits strong contrast allowing cellular, and even subcellular resolution, and detection of specific, noise-induced pathologic changes. Our results demonstrate that the round window provides a useful access to the cochlea through the middle ear, and they motivate future development of a new and efficient diagnostic tool based on two-photon micro-endoscopy.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/1.JBO.18.3.031104 DOIArticle
http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1392727PublisherArticle
Additional Information:© 2013 SPIE. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI. [DOI: 10.1117/1.JBO.18.3.031104]. Paper 12423SS received Jul. 4, 2012; revised manuscript received Sep. 11, 2012; accepted for publication Sep. 28, 2012; published online Nov. 19, 2012. We are grateful for the support of the Bertarelli Foundation.
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Funding AgencyGrant Number
Bertarelli FoundationUNSPECIFIED
Subject Keywords:two photon microscopy; cochlea; diagnosis; noise trauma
Issue or Number:3
Record Number:CaltechAUTHORS:20130904-102718969
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130904-102718969
Official Citation:Xin Yang ; Ye Pu ; Chia-Lung Hsieh ; Cheng Ai Ong ; Demetri Psaltis, et al. "Two-photon microscopy of the mouse cochlea in situ for cellular diagnosis", J. Biomed. Opt. 18(3), 031104 (Nov 19, 2012). ; http://dx.doi.org/10.1117/1.JBO.18.3.031104
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:41080
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:17 Sep 2013 20:58
Last Modified:03 Oct 2019 05:45

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