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Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development

Liebling, Michael and Forouhar, Arian S. and Wolleschensky, Ralf and Zimmermann, Bernhard and Ankerhold, Richard and Fraser, Scott E. and Gharib, Morteza and Dickinson, Mary E. (2006) Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development. Developmental Dynamics, 235 (11). pp. 2940-2948. ISSN 1058-8388. https://resolver.caltech.edu/CaltechAUTHORS:20130910-155800799

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Abstract

We report an accurate method for studying the functional dynamics of the beating embryonic zebrafish heart. The fast cardiac contraction rate and the high velocity of blood cells have made it difficult to study cellular and subcellular events relating to heart function in vivo. We have devised a dynamic three-dimensional acquisition, reconstruction, and analysis procedure by combining (1) a newly developed confocal slit-scanning microscope, (2) novel strategies for collecting and synchronizing cyclic image sequences to build volumes with high temporal and spatial resolution over the entire depth of the beating heart, and (3) data analysis and reduction protocols for the systematic extraction of quantitative information to describe phenotype and function. We have used this approach to characterize blood flow and heart efficiency by imaging fluorescent protein-expressing blood and endocardial cells as the heart develops from a tube to a multichambered organ. The methods are sufficiently robust to image tissues within the heart at cellular resolution over a wide range of ages, even when motion patterns are only quasiperiodic. These tools are generalizable to imaging and analyzing other cyclically moving structures at microscopic scales.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1002/dvdy.20926 DOIArticle
http://onlinelibrary.wiley.com/doi/10.1002/dvdy.20926/abstractPublisherArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Gharib, Morteza0000-0002-2204-9302
Additional Information:© 2006 Wiley-Liss, Inc. Accepted 12 July 2006. Published online 18 August 2006. Grant sponsor: the National Institutes of Health; Grant number: 5R01HL078694; Grant sponsor: Swiss National Science Foundation; Grant number: PBEL2-104418; Grant number: PA002-111433 We thank S. Lin for the transgenic Tg(gata1:GFP) zebrafish line and Dimitris Beis and Didier Stainier for the Tg(tie2:GFP) line. We also thank Le Trinh and Liz Jones for helpful criticisms of these studies. S.E.F, M.E.D, and M.L. were funded by the National Institutes of Health, and M.L. was funded by the Swiss National Science Foundation.
Group:GALCIT
Funders:
Funding AgencyGrant Number
NIH5R01HL078694
Swiss National Science Foundation (SNSF)PBEL2-104418
Swiss National Science Foundation (SNSF)PA002-111433
Subject Keywords:Zebrafish; heart development; hemodynamics; confocal microscopy; image reconstruction; 4D imaging
Issue or Number:11
Record Number:CaltechAUTHORS:20130910-155800799
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130910-155800799
Official Citation:Liebling, M., Forouhar, A. S., Wolleschensky, R., Zimmermann, B., Ankerhold, R., Fraser, S. E., Gharib, M. and Dickinson, M. E. (2006), Rapid three-dimensional imaging and analysis of the beating embryonic heart reveals functional changes during development. Dev. Dyn., 235: 2940–2948. doi: 10.1002/dvdy.20926
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:41225
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Sep 2013 22:00
Last Modified:03 Oct 2019 05:47

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