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Four-dimensional cardiac imaging in living embryos via postacquisition synchronization of nongated slice sequences

Liebling, Michael and Forouhar, Arian S. and Gharib, Morteza and Fraser, Scott E. and Dickinson, Mary E. (2005) Four-dimensional cardiac imaging in living embryos via postacquisition synchronization of nongated slice sequences. Journal of Biomedical Optics, 10 (5). Art. No. 054001. ISSN 1083-3668. http://resolver.caltech.edu/CaltechAUTHORS:20130911-093716805

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Abstract

Being able to acquire, visualize, and analyze 3D time series (4D data) from living embryos makes it possible to understand complex dynamic movements at early stages of embryonic development. Despite recent technological breakthroughs in 2D dynamic imaging, confocal microscopes remain quite slow at capturing optical sections at successive depths. However, when the studied motion is periodic— such as for a beating heart—a way to circumvent this problem is to acquire, successively, sets of 2D+time slice sequences at increasing depths over at least one time period and later rearrange them to recover a 3D+time sequence. In other imaging modalities at macroscopic scales, external gating signals, e.g., an electro-cardiogram, have been used to achieve proper synchronization. Since gating signals are either unavailable or cumbersome to acquire in microscopic organisms, we have developed a procedure to reconstruct volumes based solely on the information contained in the image sequences. The central part of the algorithm is a least-squares minimization of an objective criterion that depends on the similarity between the data from neighboring depths. Owing to a wavelet-based multiresolution approach, our method is robust to common confocal microscopy artifacts. We validate the procedure on both simulated data and in vivo measurements from living zebrafish embryos.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/1.2061567 DOIArticle
http://biomedicaloptics.spiedigitallibrary.org/article.aspx?articleid=1102094PublisherArticle
ORCID:
AuthorORCID
Gharib, Morteza0000-0002-2204-9302
Fraser, Scott E.0000-0002-5739-4026
Additional Information:© 2005 SPIE. Paper 05032R received Feb. 4, 2005; revised manuscript received Apr. 7, 2005; accepted for publication May 6, 2005; published online Oct. 4, 2005. We thank S. Lin for the transgenic Tg(gata1::GFP) zebrafish line. We thank Mike Tyszka for his comments on the manuscript. M. Liebling is supported by a fellowship from the Swiss National Science Foundation, PBEL2-104418.
Group:GALCIT
Funders:
Funding AgencyGrant Number
Swiss National Science Foundation (SNSF)PBEL2-104418
Subject Keywords:confocal laser scanning microscopy; dynamic cardiac imaging; nongated synchronization; registration; wavelets
Record Number:CaltechAUTHORS:20130911-093716805
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20130911-093716805
Official Citation:J. Biomed. Opt. 10(5), 054001 (February 04, 2005April 07, 2005May 06, 2005October 04, 2005). doi:10.1117/1.2061567
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:41238
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:11 Sep 2013 22:30
Last Modified:30 Sep 2016 17:35

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