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Optical imaging of the chorioretinal vasculature in the living human eye

Kim, Dae Yu and Fingler, Jeff and Zawadzki, Robert J. and Park, Susanna S. and Morse, Lawrence S. and Schwartz, Daniel M. and Fraser, Scott E. and Werner, John S. (2013) Optical imaging of the chorioretinal vasculature in the living human eye. Proceedings of the National Academy of Sciences of the United States of America, 110 (35). pp. 14354-14359. ISSN 0027-8424. PMCID PMC3761584. https://resolver.caltech.edu/CaltechAUTHORS:20130920-105036778

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Abstract

Detailed visualization of microvascular changes in the human retina is clinically limited by the capabilities of angiography imaging, a 2D fundus photograph that requires an intravenous injection of fluorescent dye. Whereas current angiography methods enable visualization of some retinal capillary detail, they do not adequately reveal the choriocapillaris or other microvascular features beneath the retina. We have developed a noninvasive microvascular imaging technique called phase-variance optical coherence tomography (pvOCT), which identifies vasculature three dimensionally through analysis of data acquired with OCT systems. The pvOCT imaging method is not only capable of generating capillary perfusion maps for the retina, but it can also use the 3D capabilities to segment the data in depth to isolate vasculature in different layers of the retina and choroid. This paper demonstrates some of the capabilities of pvOCT imaging of the anterior layers of choroidal vasculature of a healthy normal eye as well as of eyes with geographic atrophy (GA) secondary to age-related macular degeneration. The pvOCT data presented permit digital segmentation to produce 2D depth-resolved images of the retinal vasculature, the choriocapillaris, and the vessels in Sattler’s and Haller’s layers. Comparisons are presented between en face projections of pvOCT data within the superficial choroid and clinical angiography images for regions of GA. Abnormalities and vascular dropout observed within the choriocapillaris for pvOCT are compared with regional GA progression. The capability of pvOCT imaging of the microvasculature of the choriocapillaris and the anterior choroidal vasculature has the potential to become a unique tool to evaluate therapies and understand the underlying mechanisms of age-related macular degeneration progression.


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1073/pnas.1307315110 DOIArticle
http://www.pnas.org/content/110/35/14354PublisherArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3761584/PubMed CentralArticle
ORCID:
AuthorORCID
Fraser, Scott E.0000-0002-5377-0223
Additional Information:© 2013 National Academy of Sciences. Freely available online through the PNAS open access option. Edited by Napoleone Ferrara, University of California, San Diego, La Jolla, CA, and approved July 9, 2013 (received for review April 18, 2013). Published online before print August 5, 2013. We thank R. H. Grubbs (Department of Chemistry, California Institute of Technology) for helpful discussions and support and S. Garcia (Vision Science and Advanced Retinal Imaging Laboratory, University of California, Davis Medical Center) for help with phase-variance optical coherence tomography data acquisition and acquiring fundus photographs. This research was funded in part by National Eye Institute Grant EY014743 (to J.S.W.), Research to Prevent Blindness (J.S.W., S.S.P., and L.S.M.), the Beckman Institute (S.E.F.), the That Man May See Foundation (D.M.S.), and Howard Hughes Medical Institute Med-into-Grad Initiative 56006769 (to D.Y.K.). Author contributions: D.Y.K., S.E.F., and J.S.W. designed research; D.Y.K., J.F., and R.J.Z. performed research; D.Y.K., S.S.P., L.S.M., and D.M.S. analyzed data; and D.Y.K., J.F., R.J.Z., S.S.P., L.S.M., D.M.S., S.E.F., and J.S.W. wrote the paper.
Funders:
Funding AgencyGrant Number
National Eye InstituteEY014743
Research to Prevent BlindnessUNSPECIFIED
Caltech Beckman InstituteUNSPECIFIED
That Man May See FoundationUNSPECIFIED
Howard Hughes Medical Institute (HHMI)56006769
Subject Keywords:ocular circulation; ocular vasculature; optical angiography; ophthalmic imaging; Fourier-domain optical coherence tomography
Issue or Number:35
PubMed Central ID:PMC3761584
Record Number:CaltechAUTHORS:20130920-105036778
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20130920-105036778
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:41446
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:20 Sep 2013 19:16
Last Modified:03 Oct 2019 05:49

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