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Structure and function relationship of Zebrafish embryonic heart from confocal microscopy images

Moghaddam, Abbas N. and Forouhar, Arian and Liebling, Michael and Tsai, Huai-Jen and Gharib, Morteza (2006) Structure and function relationship of Zebrafish embryonic heart from confocal microscopy images. In: Medical Imaging 2006: Physiology, Function, and Structure from Medical Images. Proceedings of SPIE. No.6143. International Society for Optical Engineering , Bellingham, WA, Art. No. 614301. ISBN 0-8194-6186-5 . http://resolver.caltech.edu/CaltechAUTHORS:20130923-112227307

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Abstract

Confocal microscopy enables us to track myocytes in the embryonic zebrafish heart. The Zeiss LSM 5 Live high speed confocal microscope has been used to take optical sections (at 3 µm intervals and 151 frames per second) through a fluorescently labeled zebrafish heart at two developmental stages (26 and 34 hours post fertilization (hpf)). This data provides unique information allowing us to conjecture on the morphology and biomechanics of the developing vertebrate heart. Nevertheless, the myocytes, whose positions could be determined in a reliable manner, were located sparsely and mostly in one side of the heart tube. This difficulty was overcome using computational methods, that give longitudinal, radial and circumferential displacements of the myocytes as well as their contractile behavior. Applied strain analysis has shown that in the early embryonic heart tube, only the caudal region (near the in-flow) and another point in the middle of the tube can be active; the rest appears to be mostly passive. This statement is based on the delay between major strain and displacement which a material point experiences. Wave-like propagation of all three components of the displacement, especially in the circumferential direction, as well as the almost-periodic changes of the maximum strain support the hypothesis of helical muscle structure embedded in the tube. Changes of geometry in the embryonic heart after several hours are used to verify speculations about the structure based on the earlier images and aforementioned methods.


Item Type:Book Section
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1117/12.652779 DOIArticle
http://proceedings.spiedigitallibrary.org/proceeding.aspx?articleid=1322693PublisherArticle
Additional Information:© 2006 SPIE.
Group:GALCIT
Subject Keywords:cardiac mechanics, confocal microscopy, Embryonic heart, image-based biomechanical models, strain, structure, function
Record Number:CaltechAUTHORS:20130923-112227307
Persistent URL:http://resolver.caltech.edu/CaltechAUTHORS:20130923-112227307
Official Citation:Abbas N. Moghaddam ; Arian Forouhar ; Michael Liebling ; Huai-Jen Tsai ; Morteza Gharib; Structure and function relationship of Zebrafish embryonic heart from confocal microscopy images. Proc. SPIE 6143, Medical Imaging 2006: Physiology, Function, and Structure from Medical Images, 614301 (March 13, 2006); doi:10.1117/12.652779
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:41479
Collection:CaltechAUTHORS
Deposited By: Tony Diaz
Deposited On:23 Sep 2013 19:37
Last Modified:30 Sep 2016 17:35

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