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The Developmental Transcriptome of the Mosquito Aedes aegypti, an Invasive Species and Major Arbovirus Vector

Akbari, Omar S. and Amrhein, Henry and Williams, Brian and DiLoreto, Race and Sandler, Jeremy and Hay, Bruce A. (2013) The Developmental Transcriptome of the Mosquito Aedes aegypti, an Invasive Species and Major Arbovirus Vector. G3, 3 (9). pp. 1493-1509. ISSN 2160-1836 . PMCID PMC3755910. https://resolver.caltech.edu/CaltechAUTHORS:20131003-143259486

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Abstract

Mosquitoes are vectors of a number of important human and animal diseases. The development of novel vector control strategies requires a thorough understanding of mosquito biology. To facilitate this, we used RNA-seq to identify novel genes and provide the first high-resolution view of the transcriptome throughout development and in response to blood feeding in a mosquito vector of human disease, Aedes aegypti, the primary vector for Dengue and yellow fever. We characterized mRNA expression at 34 distinct time points throughout Aedes development, including adult somatic and germline tissues, by using polyA+ RNA-seq. We identify a total of 14,238 novel new transcribed regions corresponding to 12,597 new loci, as well as many novel transcript isoforms of previously annotated genes. Altogether these results increase the annotated fraction of the transcribed genome into long polyA+ RNAs by more than twofold. We also identified a number of patterns of shared gene expression, as well as genes and/or exons expressed sex-specifically or sex-differentially. Expression profiles of small RNAs in ovaries, early embryos, testes, and adult male and female somatic tissues also were determined, resulting in the identification of 38 new Aedes-specific miRNAs, and ~291,000 small RNA new transcribed regions, many of which are likely to be endogenous small-interfering RNAs and Piwi-interacting RNAs. Genes of potential interest for transgene-based vector control strategies also are highlighted. Our data have been incorporated into a user-friendly genome browser located at www.Aedes.caltech.edu, with relevant links to Vectorbase (www.vectorbase.org)


Item Type:Article
Related URLs:
URLURL TypeDescription
http://dx.doi.org/10.1534/g3.113.006742DOIArticle
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3755910/PubMed CentralArticle
http://www.g3journal.org/content/suppl/2013/07/04/g3.113.006742.DC1PublisherSupplemental Materials
ORCID:
AuthorORCID
Akbari, Omar S.0000-0002-6853-9884
Additional Information:© 2013 Akbari et al. Manuscript received May 10, 2013; accepted for publication June 22, 2013. This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/ by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Alexei Aravin and Katalin Fejes Toth for providing small RNA cloning protocol and expertise. We thank Lorain Schaeffer and Vijaya Kumar for help with library preparations and sequencing. This work was supported by grants to B.A.H. by the National Institutes of Health (DP1 OD003878) and the Weston Havens Foundation, the Bren foundation to Barbara Wold, the Caltech Beckman Functional Genomic Center, and the Millard and Muriel Jacobs Genetics and Genomics Laboratory at California Institute of Technology. Brian Williams and Henry Amrhein were supported by grants to Barbara Wold. Communicating editor: S. Celniker.
Group:Millard and Muriel Jacobs Genetics and Genomics Laboratory
Funders:
Funding AgencyGrant Number
NIHDP1 OD003878
Weston Havens FoundationUNSPECIFIED
Bren FoundationUNSPECIFIED
Caltech Beckman Functional Genomic CenterUNSPECIFIED
Millard and Muriel Jacobs Genetics and Genomics LaboratoryUNSPECIFIED
Subject Keywords:Aedes aegypti, dengue fever, yellow fever, chikungunya, malaria, population replacement, transcriptomes, Medea, gene drive
Issue or Number:9
PubMed Central ID:PMC3755910
Record Number:CaltechAUTHORS:20131003-143259486
Persistent URL:https://resolver.caltech.edu/CaltechAUTHORS:20131003-143259486
Official Citation:The Developmental Transcriptome of the Mosquito Aedes aegypti, an Invasive Species and Major Arbovirus Vector Omar S. Akbari, Igor Antoshechkin, Henry Amrhein, Brian Williams, Race Diloreto, Jeremy Sandler, and Bruce A. Hay G3 September 2013 3:1493-1509; Early Online July 5, 2013, doi:10.1534/g3.113.006742
Usage Policy:No commercial reproduction, distribution, display or performance rights in this work are provided.
ID Code:41662
Collection:CaltechAUTHORS
Deposited By: Ruth Sustaita
Deposited On:04 Oct 2013 17:16
Last Modified:29 Oct 2019 22:38

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